ArticleJournal of neuroinflammation2021
A new method for obtaining bankable and expandable adult-like microglia in mice.
Article in Journal of neuroinflammation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A validated method for banking mixed glial cultures that yield responsive microglia.bioRxiv : the preprint server for biology · 2025Article
- Per2 deficiency in microglia alleviates motor dysfunction by inhibiting ferroptosis in spinal cord injury.Communications biology · 2025Article
- ATP-P2XJournal of neuroinflammation · 2023Article
- A molecular characterization and clinical relevance of microglia-like cells derived from patients with panic disorder.Translational psychiatry · 2023Article
- A Step-by-step Protocol for Obtaining Mature Microglia from Mice.Bio-protocol · 2022Article
- Persistent Acidic Environment Induces Impaired Phagocytosis via ERK in Microglia.Neurochemical research · 2022Article
- Aged Microglia in Neurodegenerative Diseases: Microglia Lifespan and Culture Methods.Frontiers in aging neuroscience · 2021Review
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Authors and funding
4 authors.
Funding
Abstract
backgroundThe emerging role of microglia in neurological disorders requires a novel method for obtaining massive amounts of adult microglia. We aim to develop a new method for obtaining bankable and expandable adult-like microglia in mice.
methodsThe head neuroepithelial layer (NEL) that composed of microglial progenitor and neuroepithelial cells at mouse E13.5 was dissected and then cultured or banked. Microglia (MG) isolated from the cultured NEL by magnetic-activated cell sorting system were obtained and named NEL-MG.
resultsThe NEL included microglia progenitors that proliferate and ramify over time with neuroepithelial cells as feeder. In functional analysis, NEL-MG exhibited microglial functions, such as phagocytosis (microbeads, amyloid β, synaptosome), migration, and inflammatory response following lipopolysaccharide (LPS) stimulation. NEL was passage cultured and the NEL-MG exhibited a higher expression of microglia signature genes than the neonatal microglia, a widely used in vitro surrogate. Banking or long-term passage culture of NEL did not affect NEL-MG characteristics. Transcriptome analysis revealed that NEL-MG exhibited better conservation of microglia signature genes with a closer fidelity to freshly isolated adult microglia than neonatal microglia. NEL-MG could be re-expandable when they were plated again on neuroepithelial cells.
conclusionsThis new method effectively contributes to obtaining sufficient matured form of microglia (adult-like microglia), even when only a small number of experimental animals are available, leading to a broad application in the field of neuroscience.
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