ArticleStem cell reports2022
Cell position within human pluripotent stem cell colonies determines apical specialization via an actin cytoskeleton-based mechanism.
Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit.bioRxiv : the preprint server for biology · 2026Article
- Forces that shape the transcriptome: Linking cellular mechanosensing to mRNA splicing.The Journal of biological chemistry · 2026Review
- Accessible and cost-effective methods for patterning cell monolayers on compliant substrates.PloS one · 2026Article
- Dynamic proteome profiling of differentiating human embryonic stem cells towards cardiomyocytes.Scientific reports · 2025Article
- Integrin β1 activity controls colony morphology during human pluripotent stem cell state transitions.Stem cell reports · 2025Article
- ETVs dictate hPSC differentiation by tuning biophysical properties.Nature communications · 2025Article
- Spatiotemporal analysis of ratiometric biosensors in live multicellular spheroids using SPoRTS.Cell reports methods · 2025Article
- Metabolic patterns predispose human pluripotent stem cells to spatial organization of cell fate.Frontiers in cell and developmental biology · 2025Article
- Polarization of organoids by bioengineered symmetry breaking.IBRO neuroscience reports · 2024Article
- Arp2/3 complex activity enables nuclear YAP for naïve pluripotency of human embryonic stem cells.eLife · 2024Article
- Arp2/3 mediated dynamic lamellipodia of the hPSC colony edges promote liposome-based DNA delivery.Stem cells (Dayton, Ohio) · 2024Article
- Micro-patterned culture of iPSC-derived alveolar and airway cells distinguishes SARS-CoV-2 variants.Stem cell reports · 2024Article
- High-volume, label-free imaging for quantifying single-cell dynamics in induced pluripotent stem cell colonies.PloS one · 2024Article
- Advances in Regulating Cellular Behavior Using Micropatterns.The Yale journal of biology and medicine · 2023Review
- Loss of TJP1 disrupts gastrulation patterning and increases differentiation toward the germ cell lineage in human pluripotent stem cells.Developmental cell · 2023Article
- Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord-Like Organoid (pSCO) with Dorsoventral Organization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies.Journal of biological engineering · 2023Article
- Stable and efficient generation of functional iPSC-derived neural progenitor cell rosettes through regulation of collective cell-cell behavior.Frontiers in bioengineering and biotechnology · 2023Article
- Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells.Bioengineering (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human pluripotent stem cells (hPSCs) grow as colonies with epithelial-like features including cell polarity and position-dependent features that contribute to symmetry breaking during development. Our study provides evidence that hPSC colonies exhibit position-dependent differences in apical structures and functions. With this apical difference, edge cells were preferentially labeled with amphipathic dyes, which enabled separation of edge and center cells by fluorescence-activated cell sorting. Transcriptome comparison between center and edge cells showed differential expression of genes related to apicobasal polarization, cell migration, and endocytosis. Accordingly, different kinematics and mechanical dynamics were found between center and edge cells, and perturbed actin dynamics disrupted the position-dependent apical polarity. In addition, our dye-labeling approach could be utilized to sort out a certain cell population in differentiated micropatterned colonies. In summary, hPSC colonies have position-dependent differences in apical structures and properties, and actin dynamics appear to play an important role in the establishment of this position-dependent cell polarity.
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