ArticlePlant physiology2022
Alternative oxidase (AOX) 1a and 1d limit proline-induced oxidative stress and aid salinity recovery in Arabidopsis.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Crops exposed to extreme conditions: perspectives of gene editing to improve stress tolerance.Plant cell reports · 2026Review
- Amino Acids as Multifunctional Molecules in Plants: From Fundamental Metabolism to Precision Agriculture.Plants (Basel, Switzerland) · 2026Review
- Genome-wide identification ofFrontiers in plant science · 2026Article
- A retrotransposon insertion upstream of Arabidopsis thaliana CRK8 receptor-like kinase modulates a trade-off between pathogen defense and salt tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Selection of a Bacterial Conditioner to Improve Wheat Production Under Salinity Stress.Microorganisms · 2025Article
- Synergistic effects of drought and heat stress onPhotosynthetica · 2025Article
- Exogenous Calcium Enhances Castor Tolerance to Saline-Alkaline Stress by Regulating Antioxidant Enzyme Activity and Activating CaInternational journal of molecular sciences · 2024Article
- Genome-Wide Identification and Characterization ofPlants (Basel, Switzerland) · 2024Article
- Exploring Evolutionary Pathways and Abiotic Stress Responses through Genome-Wide Identification and Analysis of the Alternative Oxidase (AOX) Gene Family in Common Oat (International journal of molecular sciences · 2024Article
- The nonphototrophic hypocotyl 3 (NPH3) domain protein NRL5 is a trafficking-associated GTPase essential for drought resistance.Science advances · 2024Article
- PlantFUNCO: Integrative Functional Genomics Database Reveals Clues into Duplicates Divergence Evolution.Molecular biology and evolution · 2024Article
- Please, carefully, pass the P5C.Journal of experimental botany · 2024Article
- Determination of Free Proline in Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- ATP yield of plant respiration: potential, actual and unknown.Annals of botany · 2023Article
- AOX in action-making plant mitochondrial metabolism special.Plant physiology · 2023Article
- High-throughput, dynamic, multi-dimensional: an expanding repertoire of plant respiration measurements.Plant physiology · 2023Article
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Unique opportunities for future research on the alternative oxidase of plants.Plant physiology · 2023Article
- Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress.The Plant cell · 2023Article
- Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Plants (Basel, Switzerland) · 2022Article
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proline (Pro) catabolism and reactive oxygen species production have been linked in mammals and Caenorhabditis elegans, while increases in leaf respiration rate follow Pro exposure in plants. Here, we investigated how alternative oxidases (AOXs) of the mitochondrial electron transport chain accommodate the large, atypical flux resulting from Pro catabolism and limit oxidative stress during Pro breakdown in mature Arabidopsis (Arabidopsis thaliana) leaves. Following Pro treatment, AOX1a and AOX1d accumulate at transcript and protein levels, with AOX1d approaching the level of the typically dominant AOX1a isoform. We therefore sought to determine the function of both AOX isoforms under Pro respiring conditions. Oxygen consumption rate measurements in aox1a and aox1d leaves suggested these AOXs can functionally compensate for each other to establish enhanced AOX catalytic capacity in response to Pro. Generation of aox1a.aox1d lines showed complete loss of AOX proteins and activity upon Pro treatment, yet full respiratory induction in response to Pro remained possible via the cytochrome pathway. However, aox1a.aox1d leaves displayed symptoms of elevated oxidative stress and suffered increased oxidative damage during Pro metabolism compared to the wild-type (WT) or the single mutants. During recovery from salt stress, when relatively high rates of Pro catabolism occur naturally, photosynthetic rates in aox1a.aox1d recovered slower than in the WT or the single aox lines, showing that both AOX1a and AOX1d are beneficial for cellular metabolism during Pro drawdown following osmotic stress. This work provides physiological evidence of a beneficial role for AOX1a but also the less studied AOX1d isoform in allowing safe catabolism of alternative respiratory substrates like Pro.
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