Evidence map›Paper›PMID 34919167›Full record

ArticleExtremophiles : life under extreme conditions2021

The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide.

Surbhi Jain, Alexander Katsyv, Mirko Basen, Volker Müller

Open access · hybridAbstract read
In one paragraph

Article in Extremophiles : life under extreme conditions, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 33 citations in OpenAlex.

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  13. Isolation, Genomic Sequence and Physiological Characterization ofApplied and environmental microbiology · 2023
    Article
  14. Article
  15. A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023
    Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Surbhi JainDepartment of Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
Alexander KatsyvDepartment of Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
Mirko BasenMicrobiology, Institute of Biological Sciences, University of Rostock, 18059, Rostock, Germany.
Volker MüllerDepartment of Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany. vmueller@bio.uni-frankfurt.de.
Goethe University Frankfurt · DEUniversity of Rostock · DE

Funding

Bundesministerium für Bildung und Forschung ThermoSynCon/031B0857B
6 · The paper itself

Abstract

Thermoanaerobacter kivui is a thermophilic acetogen that can grow on carbon monoxide as sole carbon and energy source. To identify the gene(s) involved in CO oxidation, the genome sequence was analyzed. Two genes potentially encoding CO dehydrogenases were identified. One, cooS, potentially encodes a monofunctional CO dehydrogenase, whereas another, acsA, potentially encodes the CODH component of the CODH/ACS complex. Both genes were cloned, a His-tag encoding sequence was added, and the proteins were produced from a plasmid in T. kivui. His-AcsA copurified by affinity chromatography with AcsB, the acetyl-CoA synthase of the CO dehydrogenase/acetyl CoA synthase complex. His-CooS copurified with CooF

Indexed as

Aldehyde OxidoreductasesCarbon MonoxideMultienzyme ComplexesThermoanaerobacterAldehyde OxidoreductasesCarbon Monoxidecarbon monoxide dehydrogenaseMultienzyme ComplexesBiofuelsSynthesis gasThermophilic acetogenic bacteriaWood–Ljungdahl pathway

Identifiers

PMID34919167
PMCPMC8683389
OpenAlexW4200494204

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.