Evidence map›Paper›PMID 34918302›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer.

Dylan O Matvey, Thomas S C Ng, Miles A Miller

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Dylan O MatveyCenter for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
Thomas S C NgCenter for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
Miles A MillerCenter for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA. miles.miller@mgh.harvard.edu.
Center for Systems Biology · USHarvard University · US

Funding

Dissection of in situ myeloid signaling using image-guided synthetic controlDP2CA259675 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI MILLER, MILES A · 2020 to 2023
$2.7M
Single Cell In Vivo Imaging Technology to Analyze Taxane Nano Formulations in Breast CancerR00CA207744 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI MILLER, MILES A · 2018 to 2020
$747k
NCI NIH HHS DP2 CA259675NCI NIH HHS R00 CA207744
6 · The paper itself

Abstract

Ovarian cancer (OVCA) is frequently detected at late stages of disease, often with dissemination throughout the peritoneal cavity surface, abdomen, and ascites fluid. Tumor signaling via mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways can promote OVCA progression and depend on local microenvironmental cues. To better study OVCA in situ within native tissue contexts, here we describe confocal microscopy techniques to image mouse models of intraperitoneal disease at a single-cell resolution. As a proof of principle demonstration, examples are highlighted for simultaneously imaging tumor vascularization, infiltrating and often immunosuppressive immune cells (tumor-associated macrophages), and OVCA kinase activity.

Indexed as

Ovarian NeoplasmsAnimalsCarcinoma, Ovarian EpithelialCell Line, TumorFemaleMicePhosphatidylinositol 3-KinasesSignal TransductionPhosphatidylinositol 3-KinasesConfocal/multiphoton microscopyHigh-grade serous ovarian cancerIntravital microscopyLow-grade serous ovarian cancerPhosphosignaling

Identifiers

PMID34918302
OpenAlexW4200461300

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.