Multispectral Staining and Analysis of Extracellular Matrix.

Carine M Renner, Mike R Visetsouk, Pamela K Kreeger, Kristyn S Masters

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.5field-weighted citation impact, top 33% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Carine M RennerDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Mike R VisetsoukDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Pamela K KreegerDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA.

Kristyn S MastersDepartment of Biomedical Engineering and Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, WI, USA. kmasters@wisc.edu.

University of Wisconsin–Madison · USUniversity of Wisconsin Carbone Cancer Center · US

Funding

Engineered ECM platforms to analyze progression in high grade serous ovarian cancerR01CA232517 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI CAMPAGNOLA, PAUL J, KREEGER, PAMELA K · 2018 to 2022

$2.4M

NCI NIH HHS R01 CA232517

6 · The paper itself

Abstract

Multiplexed immunofluorescent (IF) techniques enable the detection of multiple antigens within the same sample and are therefore useful in situations where samples are rare or small in size. Similar to standard IF, multiplexed IF yields information on both the location and relative amount of detected antigens. While this method has been used primarily to detail cell phenotypes, we have recently adapted it to profile the extracellular matrix (ECM), which provides technical challenges due to autofluorescence and spatial overlap. This chapter details the planning, execution, optimization, and troubleshooting to use multiplexed IF to profile the ECM of human fallopian tube tissue.

Indexed as

Extracellular MatrixCarcinoma in SituCystadenocarcinoma, SerousFallopian Tube NeoplasmsFallopian TubesFemaleHumansOvarian NeoplasmsStaining and LabelingOvarian cancerSerous tubal intra-epithelial carcinomaSTIC

Identifiers

PMID34918289

DOI10.1007/978-1-0716-1956-8_6

PMCPMC11409815

OpenAlexW4200076927

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.