Evidence map›Paper›PMID 34918243›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Plant-Based Systems for Vaccine Production.

Mattia Santoni, Elisa Gecchele, Roberta Zampieri, Linda Avesani

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mattia SantoniDepartment of Biotechnology, University of Verona, Verona, Italy.
Elisa GeccheleDiamante srl, Verona, Italy.
Roberta ZampieriDiamante srl, Verona, Italy.
Linda AvesaniDepartment of Biotechnology, University of Verona, Verona, Italy. linda.avesani@univr.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant systems have been used as biofactories to produce recombinant proteins since 1983. The huge amount of data, collected so far in this framework, suggests that plants display several key advantages over existing traditional platforms when they are intended for therapeutic uses, including safety, scalability, and the speed in obtaining the final product.Here, we describe a method that could be applied for the expression and production of a candidate subunit vaccine in Nicotiana benthamiana plants by transient expression, defining all the protocols starting from plant cultivation to target recombinant protein purification.

Indexed as

VaccinesChromatography, AffinityNicotianaPlants, Genetically ModifiedRecombinant ProteinsRecombinant ProteinsVaccinesBiofactoriesBiopharmaceuticalDesign-of-experimentDownstream processingMolecular farmingNicotiana benthamianaTransient expression

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.