Evidence map›Paper›PMID 34917762›Full record

ArticleClinical mass spectrometry (Del Mar, Calif.)2019

Detection and characterization of TDP-43 in human cells and tissues by multiple reaction monitoring mass spectrometry.

Taylor D Pobran, Lauren M Forgrave, Yu Zi Zheng, John G K Lim, Ian R A Mackenzie, Mari L DeMarco

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical mass spectrometry (Del Mar, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Truncated TDP-43 proteoforms diagnostic of frontotemporal dementia with TDP-43 pathology.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2024
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Taylor D PobranDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Lauren M ForgraveDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Yu Zi ZhengDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
John G K LimDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Ian R A MackenzieDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
Mari L DeMarcoDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.
University of British Columbia · CAProvidence Health Care · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transactive response DNA-binding protein 43 kDa (TDP-43) is a highly conserved and widely expressed protein in human tissues that regulates nucleic acid processing. In frontotemporal dementia and amyotrophic lateral sclerosis, however, TDP-43 forms insoluble aggregates in central nervous tissues. These pathological deposits of TDP-43 have been primarily studied by ligand binding, namely western blot analysis, and, thus, methods with greater structural resolution are needed to aid in our understanding of the pathological processes associated with TDP-43 misfolding and aggregation. Toward this goal, we have developed a selective and multiplex method for the detection and characterization of TDP-43 using liquid chromatography tandem mass spectrometry. As proof-of-concept, the method was applied to the detection and characterization of TDP-43 in human cell lines and human brain tissue.

Indexed as

ALS, amyotrophic lateral sclerosisAmyotrophic lateral sclerosisBiomarkersePAR, expected peak area ratioFrontotemporal dementiaFTD, frontotemporal dementiaFTLD, frontotemporal lobar degenerationLC, liquid chromatographyMass spectrometryMRM, multiple reaction monitoringMS/MS, triple quadrupole mass spectrometerMultiple reaction monitoringp, detergent-insoluble fractionPTM, post-translational modificationrec, recombinants, detergent-soluble fractionS/N, signal to noise rationTDP-43, transactive response DNA-binding protein 43 kDaTransactive response DNA-binding protein 43 kDa

Identifiers

PMID34917762
PMCPMC8669462
OpenAlexW2963002070

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.