ArticleAsian journal of andrology
Semen microbiota in normal and leukocytospermic males.
Article in Asian journal of andrology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Is Lactobacillus spp. beneficial in human semen? Systematic review.JBRA assisted reproduction · 2025Pooled it
- Maternal and infant gut microbiome.iMeta · 2026Review
- Lifestyle factors impact sperm microbiota and are associated with biological and clinical issues in infertile patients undergoing assisted reproductive technologies, a proof-of-concept study.Scientific reports · 2026Article
- Metagenomics and metabolomics analyses of the mechanism of non-expression of natural mating behavior in captive male Malayan pangolins (Frontiers in microbiology · 2026Article
- Seminal dysbiosis in idiopathic male infertility: toward a seminal dysbiosis-inflammatory/oxidative endophenotype-SDF axis.Frontiers in endocrinology · 2026Review
- Evaluation of the semen microbiome for fertility in men with obesity using next-generation sequencing.Basic and clinical andrology · 2025Article
- Research trend on the emerging role of the microbiome in idiopathic male infertility.Antonie van Leeuwenhoek · 2025Review
- Beyond Genes: Mechanistic and Epidemiological Insights into Paternal Environmental Influence on Offspring Health.Current environmental health reports · 2025Review
- Microbial genomics: a potential toolkit for forensic investigations.Forensic science, medicine, and pathology · 2025Review
- Lower Semen Quality Among Men in the Modern Era-Is There a Role for Diet and the Microbiome?Microorganisms · 2025Review
- Comprehensive microbiome and metabolome analysis revealed the changes of semen microbial characteristics and metabolic phenotypes in patients with idiopathic oligoasthenozoospermia.Frontiers in cellular and infection microbiology · 2025Article
- State-of-the-art approaches in the investigation of human seminal bacteriome using metagenomic methods.Frontiers in reproductive health · 2025Review
- Contribution of the seminal microbiome to paternal programming.Biology of reproduction · 2024Review
- Male Tract Microbiota and Male Infertility.Cells · 2024Review
- Effect of environmental factors on seminal microbiome and impact on sperm quality.Frontiers in endocrinology · 2024Review
- New insights into the genetic predisposition of brucellosis and its effect on the gut and vaginal microbiota in goats.Scientific reports · 2023Article
- Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform.International journal of molecular sciences · 2023Article
- Human Male Genital Tract Microbiota.International journal of molecular sciences · 2023Review
- Characterization of the semen, gut, and urine microbiota in patients with different semen abnormalities.Frontiers in microbiology · 2023Article
- Mammals' sperm microbiome: current knowledge, challenges, and perspectives on metagenomics of seminal samples.Frontiers in microbiology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large numbers of microbes can be present in seminal fluid, and there are differences in the semen microbiota between normal and abnormal semen samples. To evaluate the semen microbiota in patients with leukocytospermia, 87 seminal fluid samples, including 33 samples with a normal seminal leukocyte count and 54 samples with leukocytospermia, were obtained for a cross-sectional analysis. Twenty samples with a normal seminal leukocyte count had normal sperm parameters (Control group), and 13 samples with a normal seminal leukocyte count were from asthenozoospermia patients (Ast group). However, 32 samples with leukocytospermia were from asthenozoospermia patients (LA group), and only 22 samples with leukocytospermia had normal sperm parameters (Leu group). The 16S ribosomal RNA (rRNA) gene sequencing method was used to sequence the microbiota in the seminal fluid, and multiple bioinformatics methods were utilized to analyze the data. Finally, the results showed that the worse sperm parameters were observed in the leukocytospermia-related groups. Semen microbiota analysis found that there was increased alpha diversity in the leukocytospermia-related groups. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the primary phyla in the seminal fluid. Two microbiota profiles, namely, Lactobacillus-enriched and Streptococcus-enriched groups, were identified in this study. The majority of the samples in the groups with a normal seminal leukocyte count could be categorized as Lactobacillus-enriched, whereas the majority of the leukocytospermia samples could be categorized as Streptococcus-enriched. Our study indicated that males with leukocytospermia have worse sperm parameters and a different semen microbiota composition compared to males with a normal seminal leukocyte count.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.