ArticleProceedings of the National Academy of Sciences of the United States of America2021
Bacteriophage self-counting in the presence of viral replication.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Revisiting the life cycle of temperate phages.Nature reviews. Microbiology · 2026Review
- Counting to two: how phages decide between lysis and lysogeny.bioRxiv : the preprint server for biology · 2026Article
- Single-phage profiling illuminates viral individuality during bacterial cell fate determination.Nature communications · 2026Article
- Biophysical Modeling Elucidates Mechanistic Principles for Rational Molecular Glue Design.Journal of chemical information and modeling · 2026Article
- Single-phage profiling illuminates viral individuality during cell fate determination.bioRxiv : the preprint server for biology · 2026Article
- Prophage: agent provocateur?Archives of microbiology · 2026Review
- Bacteriophage deploys a RecA-dependent nuclease to inhibit Staphylococcus aureus replication and promote phage propagation.Nucleic acids research · 2026Article
- The adaptive plasticity of temperate phageVirus evolution · 2026Article
- Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle.Molecular cell · 2025Article
- Utilizing the effectiveness of phage cocktail to combat Shigella and Salmonella infections and their polymicrobial biofilm control activity.BMC microbiology · 2025Article
- Spatial propagation of temperate phages within and among biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genomic Insights into and Lytic Potential of Native Bacteriophages M8-2 and M8-3 Against Clinically Relevant Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Novel MRSA-targeting phage MetB16: Genomic features, structural insights, and therapeutic applications.Turkish journal of biology = Turk biyoloji dergisi · 2025Article
- Fine-tuned spatiotemporal dynamics of DNA replication during phage lambda infection.Journal of virology · 2024Article
- Using bacterial population dynamics to count phages and their lysogens.Nature communications · 2024Article
- Coinfecting phages impede each other's entry into the cell.Current biology : CB · 2024Article
- The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces.PLoS genetics · 2024Article
- Lytic and temperate phage naturally coexist in a dynamic population model.The ISME journal · 2024Article
- Transcription-replication interactions reveal principles of bacterial genome regulation.Research square · 2023Article
- The Burden of Survivors: How Can Phage Infection Impact Non-Infected Bacteria?International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
When host cells are in low abundance, temperate bacteriophages opt for dormant (lysogenic) infection. Phage lambda implements this strategy by increasing the frequency of lysogeny at higher multiplicity of infection (MOI). However, it remains unclear how the phage reliably counts infecting viral genomes even as their intracellular number increases because of replication. By combining theoretical modeling with single-cell measurements of viral copy number and gene expression, we find that instead of hindering lambda's decision, replication facilitates it. In a nonreplicating mutant, viral gene expression simply scales with MOI rather than diverging into lytic (virulent) and lysogenic trajectories. A similar pattern is followed during early infection by wild-type phage. However, later in the infection, the modulation of viral replication by the decision genes amplifies the initially modest gene expression differences into divergent trajectories. Replication thus ensures the optimal decision-lysis upon single-phage infection and lysogeny at higher MOI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.