ArticleSkeletal muscle2021
ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index.
Article in Skeletal muscle, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Bioprinted vascularized soft-tissue flaps with an integrated arterial-venous loop.Cell biomaterials · 2026Article
- Critical illness impairs coordinated myogenesis: a prospective longitudinal study of skeletal muscle progenitor cell biology using serial muscle biopsies (SATELLITE study).Critical care (London, England) · 2026Observational
- Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.Polymers · 2026Article
- MyoFuse is a fully AI-based workflow for automated quantification of skeletal muscle cell fusion in vitro.Scientific reports · 2026Article
- Collagen Scaffold Viscoelasticity Regulates Muscle Cell Phenotype.Advanced healthcare materials · 2026Article
- Planar cell polarity protein Vangl2 interacts with M-cadherin and stabilizes its cell surface expression in mouse C2C12 myoblasts.Frontiers in cell and developmental biology · 2026Article
- Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow twitch muscles.Scientific reports · 2025Article
- Article
- Bioprinting Perfusable and Vascularized Skeletal Muscle Flaps for the Treatment of Volumetric Muscle Loss.Advanced healthcare materials · 2025Article
- Quantitative measurement of morphometric indicators of skeletal muscle cell behaviour and quality.Journal of the Royal Society, Interface · 2025Article
- Fluorescein-based SynNotch adaptors for regulating gene expression responses to diverse extracellular and matrix-based cues.Nature communications · 2025Article
- Combinational regenerative inductive effect of bio-adhesive hybrid hydrogels conjugated with hiPSC-derived myofibers and its derived EVs for volumetric muscle regeneration.Bioactive materials · 2025Article
- Reduced myotube diameter induced by combined inhibition of transforming growth factor-β type I receptors Acvr1b and Tgfbr1 is associated with enhanced β1-syntrophin expression.Journal of cellular physiology · 2024Article
- CCL2 signaling promotes skeletal muscle wasting in non-tumor and breast tumor models.Disease models & mechanisms · 2024Article
- Conductive Polyisocyanide Hydrogels Inhibit Fibrosis and Promote Myogenesis.ACS applied bio materials · 2024Article
- Engineering Stem Cell Fate Controlling Biomaterials to Develop Muscle Connective Tissue Layered Myofibers.Advanced functional materials · 2024Article
- Dietary pyrroloquinoline quinone hinders aging progression in male mice and D-galactose-induced cells.Frontiers in aging · 2024Article
- Identification of a muscle-specific isoform of VMA21 as a potent actor in X-linked myopathy with excessive autophagy pathogenesis.Human molecular genetics · 2023Article
- Article
- Optimal Pre-Plating Method of Chicken Satellite Cells for Cultured Meat Production.Food science of animal resources · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundMeasuring biological features of skeletal muscle cells is difficult because of their unique morphology and multinucleate nature upon differentiation. Here, we developed a new Fiji macro package called ViaFuse (that stands for viability and fusion) to measure skeletal muscle cell viability and differentiation. To test ViaFuse, we utilized immunofluorescence images of differentiated myotubes where the capping actin protein of muscle z-line subunit beta (CAPZB) was depleted in comparison with control cells.
resultsWe compared the values achieved using the ViaFuse macros first with manual quantification performed by researchers and second with those obtained utilizing the MATLAB muscle-centric software MyoCount. We observed a high degree of correlation between all methods of quantification.
conclusionsViaFuse can detect the borders of myotubes and identify nuclear clumps which have been limitations of previous muscle-centric imaging software. The ViaFuse macros require little computer power or space to run and user inputs to the ViaFuse macros are minimal, thereby automating the analysis process in a quick, easy, and accurate fashion. Additionally, the ViaFuse macros work with Fiji, an existing imaging software widely used by skeletal muscle researchers. Furthermore, ViaFuse is compatible with many computer systems, has a very intuitive interface, and does not require prior complex mathematical knowledge. Therefore, we propose ViaFuse as a robust and meticulous method to quantify skeletal muscle cell viability and differentiation.
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