ArticleBMC infectious diseases2021
The microbiological diagnostic performance of metagenomic next-generation sequencing in patients with sepsis.
Article in BMC infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Effects of a clinical metagenomics intervention on clinical outcomes, healthcare costs, and health-related quality of life in patients with sepsis or septic shock: results of the randomized-controlled DigiSep trial.Intensive care medicine · 2026Trial
- Comprehensive analysis of microorganisms in severe septic patients in the intensive care unit by mNGS and microbial culture.BMC microbiology · 2026Article
- Case Report: Cervical lymphadenitis resulting fromFrontiers in pediatrics · 2026Article
- Recent Advances and Future Perspectives in the Detection of Carbapenem-ResistantInfection and drug resistance · 2026Review
- In-matrix library preparation for metagenomic sequencing of microbial cell-free DNA.Journal of clinical microbiology · 2025Article
- Evaluating the diagnostic efficacy of metagenomic next-generation sequencing in bronchoalveolar lavage fluid for pulmonary cryptococcosis with different clinical and imaging characteristics.BMC infectious diseases · 2025Article
- Sputum metagenomics in bronchiectasis reveals pan-European variation: an EMBARC-BRIDGE study.The European respiratory journal · 2025Article
- Clinical implement of Probe-Capture Metagenomics in sepsis patients: A multicentre and prospective study.Clinical and translational medicine · 2025Article
- Presepsin, procalcitonin, interleukin-6, and high-sensitivity C-reactive protein for predicting bacterial DNAaemia among patients with sepsis.Journal of thoracic disease · 2025Article
- Exploring drug resistance genes inFrontiers in microbiology · 2025Article
- The type of pathogen is associated with organ failure and legacy dysfunction in patients with sepsis.Frontiers in immunology · 2025Article
- Advances in pediatrics in 2023: choices in allergy, analgesia, cardiology, endocrinology, gastroenterology, genetics, global health, hematology, infectious diseases, neonatology, neurology, pulmonology.Italian journal of pediatrics · 2024Review
- Comparing the diagnostic value of targeted with metagenomic next-generation sequencing in immunocompromised patients with lower respiratory tract infection.Annals of clinical microbiology and antimicrobials · 2024Article
- The clinical application value of multi-site mNGS detection of patients with sepsis in intensive care units.BMC infectious diseases · 2024Article
- Utility of paired plasma and drainage fluid mNGS in diagnosing acute intra-abdominal infections with sepsis.BMC infectious diseases · 2024Article
- Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology.Polish journal of microbiology · 2024Article
- Metagenomic next-generation sequencing of plasma cell-free DNA improves the early diagnosis of suspected infections.BMC infectious diseases · 2024Article
- Simultaneous diagnosis of tuberculous pleurisy and malignant pleural effusion using metagenomic next-generation sequencing (mNGS).Journal of translational medicine · 2023Article
- [Value of metagenomic next-generation sequencing in children with hematological malignancies complicated with infections].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2023Article
- Metagenomic next-generation sequencing for the diagnosis of oral and maxillofacial space infections.Journal of dental sciences · 2023Article
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Authors and funding
19 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIn this study, we aimed to perform a comprehensive analysis on the metagenomic next-generation sequencing for the etiological diagnosis of septic patients, and further to establish optimal read values for detecting common pathogens.
methodsIn this single-center retrospective study, septic patients who underwent pathogen detection by both microbial culture and metagenomic next-generation sequencing in the intensive care unit of the Second People's Hospital of Shenzhen from June 24, 2015, to October 20, 2019, were included.
resultsA total of 193 patients with 305 detected specimens were included in the final analysis. The results of metagenomic next-generation sequencing showed significantly higher positive rates in samples from disparate loci, including blood, bronchoalveolar lavage fluid, and cerebrospinal fluid, as well as in the determination of various pathogens. The optimal diagnostic reads were 2893, 1825.5, and 892.5 for Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae, respectively.
conclusionsThe metagenomic next-generation sequencing is capable of identifying multiple pathogens in specimens from septic patients, and shows significantly higher positive rates than culture-based diagnostics. The optimal diagnostic reads for frequently detected microbes might be useful for the clinical application of metagenomic next-generation sequencing in terms of timely and accurately determining etiological pathogens for suspected and confirmed cases of sepsis due to well-performed data interpretation.
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