ArticleBMC microbiology2021
In vitro activities of cellulase and ceftazidime, alone and in combination against Pseudomonas aeruginosa biofilms.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
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- The activity of hydrolytic enzymes and antibiotics against biofilms of bacteria isolated from industrial-scale cooling towers.Microbial cell factories · 2024Article
- Unveiling the Potential of CuO and CuNanomaterials (Basel, Switzerland) · 2024Article
- Thermothelomyces thermophilus exo- and endo-glucanases as tools for pathogenic E. coli biofilm degradation.Scientific reports · 2024Article
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- Combination antimicrobial therapy: in vitro synergistic effect of anti-staphylococcal drug oxacillin with antimicrobial peptide nisin against Staphylococcus epidermidis clinical isolates and Staphylococcus aureus biofilms.Annals of clinical microbiology and antimicrobials · 2024Article
- An apparent lack of synergy between degradative enzymes againstmicroPublication biology · 2024Article
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- Mechanisms of antimicrobial resistance in biofilms.npj antimicrobials and resistance · 2024Review
- An apparent lack of synergy between degradative enzymes againstbioRxiv : the preprint server for biology · 2023Article
- Detection of virulence genes among Staphylococcus saprophyticus isolated from women with urinary tract infections: first report from Iran.BMC research notes · 2023Article
- Strategy to combat biofilms: a focus on biofilm dispersal enzymes.NPJ biofilms and microbiomes · 2023Review
- Mathematical pharmacodynamic modeling for antimicrobial assessment of ceftazidime/colistin versus gentamicin/meropenem combinations against carbapenem-resistant Pseudomonas aeruginosa biofilm.Annals of clinical microbiology and antimicrobials · 2023Article
- Disruption of Established Bacterial and Fungal Biofilms by a Blend of Enzymes and Botanical Extracts.Journal of microbiology and biotechnology · 2023Article
- How Three Self-Secreted Biofilm Exopolysaccharides ofInternational journal of molecular sciences · 2023Review
- [Levofloxacin combined with cellulase can eradicate bacille Calmette-Guerin biofilm infection].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBiofilms are a main pathogenicity feature of Pseudomonas aeruginosa and has a significant role in antibiotic resistance and persistent infections in humans. We investigated the in vitro activities of antibiotic ceftazidime and enzyme cellulase, either alone or in combination against biofilms of P. aeruginosa.
resultsBoth ceftazidime and cellulase significantly decreased biofilm formation in all strains in a dose-dependent manner. Combination of enzyme at concentrations of 1.25, 2.5, 5, and 10 U/mL tested with 1/16× MIC of antibiotic led to a significant reduction in biofilm biomass. Cellulase showed a significant detachment effect on biofilms at three concentrations of 10 U/mL, 5 U/mL, and 2.5 U/mL. The MIC, MBC, and MBEC values of ceftazidime were 2 to 4 µg/mL, 4 to 8 µg/mL, and 2048 to 8192 µg/mL. When combined with cellulase, the MBECs of antibiotic showed a significant decrease from 32- to 128-fold.
conclusionsCombination of the ceftazidime and the cellulase had significant anti-biofilm effects, including inhibition of biofilm formation and biofilm eradication in P. aeruginosa. These data suggest that glycoside hydrolase therapy as a novel strategy has the potential to enhance the efficacy of antibiotics and helps to resolve biofilm-associated wound infections caused by this pathogen.
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