ArticlePloS one2021
Whole-genome based strain identification of fowlpox virus directly from cutaneous tissue and propagated virus.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- First Complete Genome Sequencing of a Pigeonpox Virus Strain from Mainland China and Preliminary Evaluation of Its Attenuated Potential.Veterinary sciences · 2026Article
- Genome sequence of fowlpox virus from a field outbreak in Bangladesh.Microbiology resource announcements · 2025Article
- Molecular characterization of fowl and pigeon pox viruses: A recent outbreak in Libya.Open veterinary journal · 2025Article
- First Complete Genome of Reticuloendotheliosis Virus in a Mallard Duck from Brazil: Phylogenetic Insights and Evolutionary Analysis.Pathogens (Basel, Switzerland) · 2025Article
- Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025Review
- Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (Animals : an open access journal from MDPI · 2024Article
- High-throughput sequencing as a potential tool in the quality control of infectious bronchitis vaccines.Journal of veterinary research · 2024Article
- Snapshot of the Phylogenetic Relationships among Avian Poxviruses Circulating in Portugal between 2017 and 2023.Veterinary sciences · 2023Article
- Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022.Emerging microbes & infections · 2023Article
- A novel pathogenic avipoxvirus infecting oriental turtle dove (Microbiology spectrum · 2023Article
- Complete genome analysis of African swine fever virus genotypes II, IX and XV from domestic pigs in Tanzania.Scientific reports · 2023Article
- Genetic Diversity of Fowlpox Virus and Putative Genes Involved in Its Pathogenicity.Microbiology spectrum · 2022Article
- Molecular characterisation of a novel pathogenic avipoxvirus from an Australian little crow (Corvus bennetti) directly from the clinical sample.Scientific reports · 2022Article
- Evidence of a Possible Viral Host Switch Event in an Avipoxvirus Isolated from an Endangered Northern Royal Albatross (Viruses · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fowlpox (FP) is an economically important viral disease of commercial poultry. The fowlpox virus (FPV) is primarily characterised by immunoblotting, restriction enzyme analysis in combination with PCR, and/or nucleotide sequencing of amplicons. Whole-genome sequencing (WGS) of FPV directly from clinical specimens prevents the risk of potential genome modifications associated with in vitro culturing of the virus. Only one study has sequenced FPV genomes directly from clinical samples using Nanopore sequencing, however, the study didn't compare the sequences against Illumina sequencing or laboratory propagated sequences. Here, the suitability of WGS for strain identification of FPV directly from cutaneous tissue was evaluated, using a combination of Illumina and Nanopore sequencing technologies. Sequencing results were compared with the sequence obtained from FPV grown in chorioallantoic membranes (CAMs) of chicken embryos. Complete genome sequence of FPV was obtained directly from affected comb tissue using a map to reference approach. FPV sequence from cutaneous tissue was highly similar to that of the virus grown in CAMs with a nucleotide identity of 99.8%. Detailed polymorphism analysis revealed the presence of a highly comparable number of single nucleotide polymorphisms (SNPs) in the two sequences when compared to the reference genome, providing essentially the same strain identification information. Comparative genome analysis of the map to reference consensus sequences from the two genomes revealed that this field isolate had the highest nucleotide identity of 99.5% with an FPV strain from the USA (Fowlpox virus isolate, FWPV-MN00.2, MH709124) and 98.8% identity with the Australian FPV vaccine strain (FWPV-S, MW142017). Sequencing results showed that WGS directly from cutaneous tissues is not only rapid and cost-effective but also provides essentially the same strain identification information as in-vitro grown virus, thus circumventing in vitro culturing.
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