Evidence map›Paper›PMID 34914633›Full record

ArticleJCI insight2022

Stromal architecture directs early dissemination in pancreatic ductal adenocarcinoma.

Arja Ray, Mackenzie K Callaway, Nelson J Rodríguez-Merced, Alexandra L Crampton, Marjorie Carlson, Kenneth B Emme, Ethan A Ensminger, Alexander A Kinne, Jonathan H Schrope, Haley R Rasmussen and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
4.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 62 citations in OpenAlex.

  1. Article
  2. Spatial Configuration of Pancreatic Cancer Is Associated with Disease Recurrence after Neoadjuvant Therapy and Curative-Intent Resection.Clinical cancer research : an official journal of the American Association for Cancer Research · 2026
    Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Targeting Lysosomes for Enhanced Anti-Cancer Therapeutics and Immune Response.International journal of biological sciences · 2026
    Review
  14. Extracellular matrix architecture promotes immunosuppressive microenvironments in pancreatic cancer.Matrix biology : journal of the International Society for Matrix Biology · 2025
    Article
  15. Polychromatic Polarization Microscopy Differentiates Collagen Fiber Signatures in Benign Pancreatic Tissue and Pancreatic Ductal Adenocarcinoma.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2025
    Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Arja RayDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Mackenzie K CallawayDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Nelson J Rodríguez-MercedDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Alexandra L CramptonDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Marjorie CarlsonDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Kenneth B EmmeDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Ethan A EnsmingerDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Alexander A KinneDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Jonathan H SchropeDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Haley R RasmussenDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Hong JiangDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
David G DeNardoDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
David K WoodDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
Paolo P ProvenzanoDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, Minnesota, USA.
University of Minnesota · USWashington University in St. Louis · US

Funding

Project 2: Cell Migration in Mechanically Complex MicroenvironmentsU54CA210190 · NCI · UNIVERSITY OF MINNESOTA · PI WOOD, DAVID KEVIN · 2016 to 2020
$10.0M
The Impact of Macrophage Origin on the Pathogenesis and Treatment Resistance of Pancreatic CancerR01CA177670 · NCI · WASHINGTON UNIVERSITY · PI DENARDO, DAVID G · 2014 to 2023
$3.3M
A platform to functionally sort and analyze tumor cells within combinatorial metastatic micorenvironmentsR01CA245550 · NCI · UNIVERSITY OF MINNESOTA · PI PROVENZANO, PAOLO, WOOD, DAVID KEVIN · 2020 to 2024
$2.9M
Re-wiring PDAC Tumor Immunity Through Dendritic CellsR01CA262506 · NCI · WASHINGTON UNIVERSITY · PI DENARDO, DAVID G, DESELM, CARL · 2021 to 2025
$2.8M
Targeting Focal Adhesion Kinase to Improve RT-inducted Tumor ImmunityR01CA248917 · NCI · WASHINGTON UNIVERSITY · PI DENARDO, DAVID G, KIM, HYUN · 2020 to 2024
$2.5M
Exploiting Integrin Signaling to Overcome Resistance to ImmunotherapyR01CA244938 · NCI · WASHINGTON UNIVERSITY · PI DENARDO, DAVID G, GUPTA, VINEET · 2020 to 2024
$2.2M
Stellate cells and their progenitor precursors in pancreas cancer progressionR01CA181385 · NCI · UNIVERSITY OF MINNESOTA · PI PROVENZANO, PAOLO · 2014 to 2018
$1.8M
NCI NIH HHS R01 CA177670NCI NIH HHS R01 CA181385NCI NIH HHS R01 CA244938NCI NIH HHS R01 CA245550NCI NIH HHS R01 CA248917NCI NIH HHS R01 CA262506NCI NIH HHS U54 CA210190
6 · The paper itself

Abstract

Pancreatic ductal adenocarcinoma (PDA) is an extremely metastatic and lethal disease. Here, in both murine and human PDA, we demonstrate that extracellular matrix architecture regulates cell extrusion and subsequent invasion from intact ductal structures through tumor-associated collagen signatures (TACS). This results in early dissemination from histologically premalignant lesions and continual invasion from well-differentiated disease, and it suggests TACS as a biomarker to aid in the pathologic assessment of early disease. Furthermore, we show that pancreatitis results in invasion-conducive architectures, thus priming the stroma prior to malignant disease. Analysis in potentially novel microfluidic-derived microtissues and in vivo demonstrates decreased extrusion and invasion following focal adhesion kinase (FAK) inhibition, consistent with decreased metastasis. Thus, data suggest that targeting FAK or strategies to reengineer and normalize tumor microenvironments may have roles not only in very early disease, but also for limiting continued dissemination from unresectable disease. Likewise, it may be beneficial to employ stroma-targeting strategies to resolve precursor diseases such as pancreatitis in order to remove stromal architectures that increase risk for early dissemination.

Indexed as

Gene Expression Regulation, NeoplasticNeoplasms, ExperimentalAnimalsApoptosisCarcinoma, Pancreatic DuctalCell Line, TumorCell MovementFocal Adhesion Kinase 1HumansMiceMice, TransgenicPancreatic NeoplasmsRNA, Small InterferingTumor MicroenvironmentFocal Adhesion Kinase 1Ptk2 protein, mouseRNA, Small InterferingCancerCell BiologyCell migration/adhesionDiagnostic imagingOncology

Identifiers

PMID34914633
PMCPMC8855836
OpenAlexW4220699131

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.