Evidence map›Paper›PMID 34914067›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Plasmodium falciparum Antigen Expression in Leishmania Parasite: A Way Forward for Live Attenuated Vaccine Development.

Akriti Srivastava, Swati Garg, Sweta Karan, Shikha Kaushik, Anand Ranganathan, Soumya Pati, Lalit C Garg, Shailja Singh

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Akriti SrivastavaDepartment of Life Science, School of Natural Sciences, Shiv Nadar University, Dadri, UP, India.
Swati GargSpecial Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India.
Sweta KaranGene Regulation Laboratory, National Institute of Immunology, New Delhi, India.
Shikha KaushikSpecial Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India.
Anand RanganathanSpecial Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India.
Soumya PatiDepartment of Life Science, School of Natural Sciences, Shiv Nadar University, Dadri, UP, India.
Lalit C GargGene Regulation Laboratory, National Institute of Immunology, New Delhi, India. lalit@nii.ac.in.
Shailja SinghSpecial Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India. shailja.jnu@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Live attenuated vaccines (LAVs) are among the most critical interventions in modern medicine and have already proven their potential to save millions of lives. LAVs are always explored as potential vaccine candidates since they induce an immune response, which is as good as the wild type pathogen. For parasitic diseases, the efficacy of LAVs is still under investigation and needs extensive research to mark their presence in the field. In malaria, live attenuated sporozoites have been evaluated for a vaccine against the liver stage. This vaccine approach is limited due to the highly cumbersome technique of sporozoite isolation and related relapse issues. We have developed a novel vaccine against malaria by expressing Plasmodium falciparum antigens in Leishmania donovani promastigotes. These hybrid, recombinant L. donovani parasites mimicking P. falciparum parasite antigens were analyzed for their anti-malarial efficacy in preclinical studies. We demonstrate the potential of Leishmania spp. parasites in developing an important live vector vaccine against malaria for the induction of protective immune responses. Herein, we describe a method to express malaria parasite antigens in L. donovani promastigotes and analyze its potential for a vaccine against malaria. This methodology can be extended to live, attenuated Leishmania promastigotes parasites to develop LAV against malaria.

Indexed as

Malaria, FalciparumMalaria VaccinesPlasmodium falciparumAnimalsAntigens, ProtozoanLeishmania donovaniParasitesSporozoitesVaccine DevelopmentVaccines, AttenuatedAntigens, ProtozoanMalaria VaccinesVaccines, AttenuatedL. donovaniLive attenuated vaccineMalariaPfAARPPlasmodium falciparumRecombinant parasite

Identifiers

PMID34914067

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.