Evidence map›Paper›PMID 34913130›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Salivary Gland Development in Culture.

Marcia Gaete, Tathyane H N Teshima, Lemonia Chatzeli, Abigail S Tucker

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marcia GaeteDepartment of Anatomy, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. mgaets@uc.cl.
Tathyane H N TeshimaDepartment of Oral Medicine, Eastman Dental Institute, University College London, London, UK.
Lemonia ChatzeliWellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
Abigail S TuckerCentre for Craniofacial and Regenerative Biology, King's College London, London, UK.

Funding

Department of HealthMedical Research Council MR/M026426/1
6 · The paper itself

Abstract

Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture , allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse , snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis.

Indexed as

Salivary GlandsAnimalsEpithelial CellsEpitheliumMesodermMiceMorphogenesisOrgan Culture TechniquesSubmandibular GlandBranching morphogenesisCleftingHuman organ cultureSalivary glandSnake organ culture

Identifiers

PMID34913130

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.