ArticlePhotosynthesis research2022
Mass spectrometry analysis of the photosystem II assembly factor Psb27 revealed variations in its lipid modification.
Article in Photosynthesis research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Structural interactions of TLP18.3 and Psb27-H1 to the luminal CP43 and rubredoxin-ENH1 to the stromal side of photosystem II in higher plants.The Journal of biological chemistry · 2026Article
- Integrated morphological, physiological, and transcriptomic profiling unveils a root-centric defense network mediating aluminum tolerance in Trifolium repens L.BMC plant biology · 2026Article
- Thermophilic cyanobacteria-exciting, yet challenging biotechnological chassis.Applied microbiology and biotechnology · 2024Review
- The increasing role of structural proteomics in cyanobacteria.Essays in biochemistry · 2023Review
- Large-scale top-down proteomics of the Arabidopsis thaliana leaf and chloroplast proteomes.Proteomics · 2023Article
- Advances in the Understanding of the Lifecycle of Photosystem II.Microorganisms · 2022Review
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Authors and funding
4 authors.
Funding
Abstract
The assembly of large, multi-cofactor membrane protein complexes like photosystem II (PSII) requires a high level of coordination. The process is facilitated by a large network of auxiliary proteins that bind transiently to unassembled subunits, preassembled modules or intermediate states of PSII, which are comprised of a subset of subunits. However, analysis of these immature, partially assembled PSII complexes is hampered by their low abundance and intrinsic instability. In this study, PSII was purified from the thermophilic cyanobacterium Thermosynechococcus elongatus via Twin-Strep-tagged CP43 and further separated by ion exchange chromatography into mature and immature complexes. Mass spectrometry analysis of the immature Psb27-PSII intermediate revealed six different Psb27 proteoforms with distinct lipid modifications. The maturation and functional role of thylakoid localized lipoproteins are discussed.
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