Evidence map›Paper›PMID 34909756›Full record

ArticleMedComm2021

SARS-CoV-2 spike protein harnesses SNX27-mediated endocytic recycling pathway.

Lin Zhao, Kunhong Zhong, Jia Zhao, Xin Yong, Aiping Tong, Da Jia

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MedComm, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
  2. Article
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  4. Review
  5. Article
  6. FAM91A1-TBC1D23 complex structure reveals human genetic variations susceptible for PCH.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  7. TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Article
  8. Article
  9. ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  10. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lin ZhaoKey Laboratory of Birth Defects and Related Diseases of Women and Children Department of Paediatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy West China Second University Hospital Sichuan University Chengdu China.
Kunhong ZhongState Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University Chengdu China.
Jia ZhaoKey Laboratory of Birth Defects and Related Diseases of Women and Children Department of Paediatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy West China Second University Hospital Sichuan University Chengdu China.
Xin YongKey Laboratory of Birth Defects and Related Diseases of Women and Children Department of Paediatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy West China Second University Hospital Sichuan University Chengdu China.
Aiping TongState Key Laboratory of Biotherapy and Cancer Center West China Hospital West China Medical School Sichuan University Chengdu China.
Da JiaKey Laboratory of Birth Defects and Related Diseases of Women and Children Department of Paediatrics State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy West China Second University Hospital Sichuan University Chengdu China.ORCID https://orcid.org/0000-0002-2205-1998
Sichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SARS-CoV-2 is an enveloped positive-sense RNA virus that depends on host factors for all stages of its life. Membrane receptor ACE2 is a well-established factor for SARS-CoV-2 docking. In addition to ACE2, whole-genome genetic screens have identified additional proteins, such as endosomal trafficking regulators SNX27 and retromer, as key host factors required for SARS-CoV-2 infection. However, it is poorly understood how SARS-CoV-2 utilize host endocytic transport pathways to produce productive infection. Here, we report that SNX27 interacts with the SARS-CoV-2 spike (S) protein to facilitate S protein surface expression. Interestingly, S protein binds to the PDZ domain of SNX27, although it does not contain a PDZ-binding motif (PDZbm). Either abrogation of the SNX27 PDZ domain or S protein "MTSC" motif, which is critical for SNX27 binding, decreases surface expression of S protein and viral production. Collectively, our study highlights a novel approach utilized by SARS-CoV-2 to facilitate virion trafficking to establish virus infection.

Indexed as

endocytic traffickingendosomehost–pathogen interactionSARS‐CoV‐2S protein

Identifiers

PMID34909756
PMCPMC8661858
OpenAlexW3203205149

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.