ArticleBio-protocol2021
Isolation of Primary Leydig Cells from Murine Testis.
Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- DNMT1 Regulates Testosterone Synthesis in Aged Rat Leydig Cells via FTO-Mediated HSP90AA1 mAging cell · 2026Article
- Hexaconazole-Induced Male Reproductive Toxicity Through ROS-Mediated Ferroptosis and Impaired Leydig Cell Steroidogenesis.International journal of molecular sciences · 2026Article
- Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2.iScience · 2026Article
- Branched chain amino acid sufficiency is necessary for proper luteinizing hormone response and testosterone synthesis.Reproductive biology · 2026Article
- CDK5RAP3 Regulates Testosterone Production in Mouse Leydig Cells.International journal of molecular sciences · 2026Article
- Article
- Characterization of Metabolic Patterns in Mouse Spermatogenesis and Its Clinical Implications in Humans.International journal of molecular sciences · 2025Article
- Molecular characterization of the murine Leydig cell lines TM3 and MLTC-1.Frontiers in endocrinology · 2025Article
- The role of Sertoli cell-derived miR-143-3p in male fertility declines with age.Molecular therapy. Nucleic acids · 2024Article
- The mitochondrial protease PARL is required for spermatogenesis.Communications biology · 2024Article
- Role of miR-300-3p in Leydig cell function and differentiation: A therapeutic target for obesity-related testosterone deficiency.Molecular therapy. Nucleic acids · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In males, Leydig cells are the primary source of testosterone, which is necessary for testis development, masculinization, and spermatogenesis. Leydig cells are a valuable cellular model for basic research; thus, it is important to develop an improved method for isolation and purification of Leydig cells from testes. The available methods for Leydig cell isolation have some drawbacks, including the need for sophisticated instruments, high cost, tediousness, and time consumption. Here, we describe an improved protocol for isolation of primary Leydig cells from testicular tissue by digestion with collagenase IV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.