ArticleMicrobiology spectrum2021
Evaluation of Sample Preservation and Storage Methods for Metaproteomics Analysis of Intestinal Microbiomes.
Article in Microbiology spectrum, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Optimized approaches to profile low biomass DNA and RNA virome in the upper respiratory tract of beef cattle.Animal microbiome · 2026Article
- Differential proteomics of bacteria grownmSystems · 2026Article
- The need for standardization and improved open (meta)data practices in metaproteomics.Microbiome · 2026Article
- Assessing the diversity and functional profile of the "microbial proteome" in fermented foods.Food & function · 2026Article
- Adaptations in gut Bacteroidales facilitate stable co-existence with their lytic bacteriophages.Gut microbes · 2025Article
- Article
- Assessing the diversity and functional profile of the "microbial proteome" in fermented foods.bioRxiv : the preprint server for biology · 2025Article
- Metaproteomic Analysis to Assess the Impact of Storage Media on Human Gut Microbiome in Fecal Samples.Proteomics · 2025Article
- Large Quantities of Bacterial DNA and Protein in Common Dietary Protein Source Used in Microbiome Studies.Proteomics · 2025Article
- Dietary protein from different sources escapes host digestion and is differentially modified by gut microbiota.bioRxiv : the preprint server for biology · 2025Article
- The microbiologist's guide to metaproteomics.iMeta · 2025Review
- High-throughput workflow for cultivation and characterization of gut microbiota strains with anti-inflammatory properties and metabolite signature associated with gut-brain communication.Scientific reports · 2025Article
- Source of dietary protein alters the abundance of proteases, intestinal epithelial and immune proteins both directly and via interactions with the gut microbiota.bioRxiv : the preprint server for biology · 2025Article
- Dietary protein source alters gut microbiota composition and function.The ISME journal · 2025Article
- Metaproteomics-based stable isotope fingerprinting links intestinal bacteria to their carbon source and captures diet-induced substrate switching.The ISME journal · 2025Article
- Article
- Chinese expert consensus on standard technical specifications for a gut microecomics laboratory (Review).Experimental and therapeutic medicine · 2024Review
- Metaproteomics and DNA metabarcoding as tools to assess dietary intake in humans.bioRxiv : the preprint server for biology · 2024Article
- Metabolically-versatile Ca. Thiodiazotropha symbionts of the deep-sea lucinid clamISME communications · 2024Article
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A critical step in studies of the intestinal microbiome using meta-omics approaches is the preservation of samples before analysis. Preservation is essential for approaches that measure gene expression, such as metaproteomics, which is used to identify and quantify proteins in microbiomes. Intestinal microbiome samples are typically stored by flash-freezing and storage at -80°C, but some experimental setups do not allow for immediate freezing of samples. In this study, we evaluated methods to preserve fecal microbiome samples for metaproteomics analyses when flash-freezing is not possible. We collected fecal samples from C57BL/6 mice and stored them for 1 and 4 weeks using the following methods: flash-freezing in liquid nitrogen, immersion in RNA
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