ArticleParasite (Paris, France)2021
Point of care colourimetric and lateral flow LAMP assay for the detection of Haemonchus contortus in ruminant faecal samples.
Article in Parasite (Paris, France), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- Discriminating bovine mastitis pathogens by combining loop-mediated isothermal amplification and amplicon-binding split trehalase assay.Frontiers in veterinary science · 2024Article
- Understanding theFrontiers in parasitology · 2024Review
- Development of a loop-mediated isothermal amplification detection assay forFrontiers in veterinary science · 2024Article
- COMBAR - Combatting anthelmintic resistance in ruminants.Parasite (Paris, France) · 2023Article
- Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality.Bioengineering (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Košice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main rate-limiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC
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Registered trials
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