ArticleNature communications2021
A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Protein Engineering-Enabled Cryo-EM Investigation of Small GTPases.Journal of molecular biology · 2026Article
- Integrating multitemperature sample preparation with particle rebalancing addresses preferred orientation.PNAS nexus · 2026Article
- Diversifying particle-orientation distributions in cryo-EM with LEA protein additives.bioRxiv : the preprint server for biology · 2026Article
- UsingbioRxiv : the preprint server for biology · 2026Article
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification.bioRxiv : the preprint server for biology · 2025Article
- Foam film vitrification for cryo-EM.Nature communications · 2025Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- A strategic approach for efficient cryo-EM grid optimization using design of experiments.Journal of structural biology · 2025Article
- Overcoming the preferred-orientation problem in cryo-EM with self-supervised deep learning.Nature methods · 2025Article
- Article
- Self-assembled superstructure alleviates air-water interface effect in cryo-EM.Nature communications · 2024Article
- Factors affecting macromolecule orientations in thin films formed in cryo-EM.Acta crystallographica. Section D, Structural biology · 2024Article
- Overcoming the preferred orientation problem in cryoEM with self-supervised deep-learning.bioRxiv : the preprint server for biology · 2024Article
- Cryo-EM Map Anisotropy Can Be Attenuated by Map Post-Processing and a New Method for Its Estimation.International journal of molecular sciences · 2024Article
- Identification and Characterization of a Predominant Hydrophobin in the Edible MushroomJournal of fungi (Basel, Switzerland) · 2023Article
- Diversity of hydrodynamic radii of intrinsically disordered proteins.European biophysics journal : EBJ · 2023Article
- Theoretical framework and experimental solution for the air-water interface adsorption problem in cryoEM.Biophysics reports · 2023Article
- cryoWriter: a blotting free cryo-EM preparation system with a climate jet and cover-slip injector.Faraday discussions · 2022Article
- Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.Acta crystallographica. Section D, Structural biology · 2022Article
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9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution structures of biomacromolecules in solution. However, air-water interface induced preferred orientations, dissociation or denaturation of biomacromolecules during cryo-vitrification remains a limiting factor for many specimens. To solve this bottleneck, we developed a cryo-EM support film using 2D crystals of hydrophobin HFBI. The hydrophilic side of the HFBI film adsorbs protein particles via electrostatic interactions and sequesters them from the air-water interface, allowing the formation of sufficiently thin ice for high-quality data collection. The particle orientation distribution can be regulated by adjusting the buffer pH. Using this support, we determined the cryo-EM structures of catalase (2.29 Å) and influenza haemagglutinin trimer (2.56 Å), which exhibited strong preferred orientations using a conventional cryo-vitrification protocol. We further show that the HFBI film is suitable to obtain high-resolution structures of small proteins, including aldolase (150 kDa, 3.28 Å) and haemoglobin (64 kDa, 3.6 Å). Our work suggests that HFBI films may have broad future applications in increasing the success rate and efficiency of cryo-EM.
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