ArticleProceedings of the National Academy of Sciences of the United States of America2021
The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 38 citations in OpenAlex.
- Arabidopsis ECHIDNA Plays an Important Role in Auxin Regulation of Clathrin-Mediated Trafficking During Root Gravitropism.Plant, cell & environment · 2026Article
- Direct coupling of nod factor signaling to vesicular trafficking initiates legume nodulation.Nature communications · 2026Article
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Clathrin-mediated endocytosis of ERECTA family receptors is essential for proper stomatal development inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- A combined biochemical and computational approach provides evidence for membrane remodelling by the structural scaffold of the endocytic TPLATE complex.Nature plants · 2025Article
- Prevalence and environmental abundance of the TSET complex in cosmopolitan algal groups.iScience · 2025Article
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana.Journal of cell science · 2024Article
- TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance.EMBO reports · 2023Article
- Protein phase separation in plant membrane biology: more than just a compartmentalization strategy.The Plant cell · 2023Article
- Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator.Nature plants · 2023Article
- Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components.The Plant cell · 2022Article
- Molecular mechanisms of endomembrane trafficking in plants.The Plant cell · 2022Review
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- An Update on Coat Protein Complexes for Vesicle Formation in Plant Post-Golgi Trafficking.Frontiers in plant science · 2022Review
- Compromised osteogenic effect of exosomes internalized by senescent bone marrow stem cellsFrontiers in bioengineering and biotechnology · 2022Article
- Local endocytosis of sucrose transporter 2 in duckweed reveals the role of sucrose transporter 2 in guard cells.Frontiers in plant science · 2022Article
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Authors and funding
15 authors at 4 institutions in 4 countries.
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Abstract
Clathrin-mediated endocytosis is the major route of entry of cargos into cells and thus underpins many physiological processes. During endocytosis, an area of flat membrane is remodeled by proteins to create a spherical vesicle against intracellular forces. The protein machinery which mediates this membrane bending in plants is unknown. However, it is known that plant endocytosis is actin independent, thus indicating that plants utilize a unique mechanism to mediate membrane bending against high-turgor pressure compared to other model systems. Here, we investigate the TPLATE complex, a plant-specific endocytosis protein complex. It has been thought to function as a classical adaptor functioning underneath the clathrin coat. However, by using biochemical and advanced live microscopy approaches, we found that TPLATE is peripherally associated with clathrin-coated vesicles and localizes at the rim of endocytosis events. As this localization is more fitting to the protein machinery involved in membrane bending during endocytosis, we examined cells in which the TPLATE complex was disrupted and found that the clathrin structures present as flat patches. This suggests a requirement of the TPLATE complex for membrane bending during plant clathrin-mediated endocytosis. Next, we used in vitro biophysical assays to confirm that the TPLATE complex possesses protein domains with intrinsic membrane remodeling activity. These results redefine the role of the TPLATE complex and implicate it as a key component of the evolutionarily distinct plant endocytosis mechanism, which mediates endocytic membrane bending against the high-turgor pressure in plant cells.
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