Evidence map›Paper›PMID 34906050›Full record

ArticleBiochemistry. Biokhimiia2021

Monoclonal Antibody Targeting the HA191/199 Region of H1N1 Influenza Virus Mediates the Damage of Neural Cells.

Chun-Yan Guo, Qing Feng, Li-Ting Yan, Xin Xie, Dao-Yan Liang, Yan Li, Yang-Meng Feng, Li-Jun Sun, Jun Hu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry. Biokhimiia, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Chun-Yan GuoCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. guochunyan101109@126.com.
Qing FengCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.
Li-Ting YanCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.
Xin XieKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi, 710069, China.
Dao-Yan LiangCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.
Yan LiCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.
Yang-Meng FengCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China.
Li-Jun SunCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. lijun200206@163.com.
Jun HuCentral Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, 710068, China. 78364282@qq.com.
Shaanxi Provincial People's Hospital · CNShanxi Medical University · CNMinistry of Education of the People's Republic of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vaccination is the most effective mean of preventing influenza virus infections. However, vaccination-induced adverse reactions of the nervous system, the causes of which are unknown, lead to concerns on the safety of influenza A vaccine. In this study, we used flow cytometry, cell ELISA, and immunofluorescence to find that H1-84 monoclonal antibody (mAb) against the191/199 region of the H1N1 influenza virus hemagglutinin (HA) protein binds to neural cells and mediates cell damage. Using molecular simulation software, such as PyMOL and PDB viewer, we demonstrated that the HA191/199 region maintains the overall structure of the HA head. Since the HA191/199 region cannot be removed from the HA structure, it has to be altered via introducing point mutations by site-directed mutagenesis. This will provide an innovative theoretical support for the subsequent modification the influenza A vaccine for increasing its safety.

Indexed as

Antibodies, Monoclonal, Murine-DerivedHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H1N1 SubtypeMolecular Dynamics SimulationAntibodies, ViralCell Line, TumorHumansMutagenesis, Site-DirectedNeuronsProtein DomainsAntibodies, Monoclonal, Murine-DerivedAntibodies, ViralH1N1 virus hemagglutininHemagglutinin Glycoproteins, Influenza VirusHA191/199 regioninfluenza A virusmonoclonal antibodies (mAbs)neuronal cell damage

Identifiers

PMID34906050
PMCPMC8588936
OpenAlexW3211400675

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.