Evidence map›Paper›PMID 34903829›Full record

ArticleScientific reports2021

Cy3-ATP labeling of unfixed, permeabilized mouse hair cells.

Itallia V Pacentine, Peter G Barr-Gillespie

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Myosin motors in sensory hair bundle assembly.Current opinion in cell biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Itallia V PacentineOregon Hearing Research Center & Vollum Institute, Mail Code L335A, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR, 97239, USA.
Peter G Barr-GillespieOregon Hearing Research Center & Vollum Institute, Mail Code L335A, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR, 97239, USA. gillespp@ohsu.edu.
Oregon Health & Science University · USVollum Institute · US

Funding

Hair-Bundle ProteinsR01DC002368 · NIDCD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Peter Gordon Barr-Gillespie · 1994 to 2026
$11.3M
Functions of Myosin VII in Mechanosensory Hair CellsR01DC014427 · NIDCD · OREGON HEALTH & SCIENCE UNIVERSITY · PI BARR-GILLESPIE, PETER GORDON, MUELLER, ULRICH · 2016 to 2020
$3.1M
NIDCD NIH HHS R01 DC002368NIDCD NIH HHS R01 DC014427
6 · The paper itself

Abstract

ATP-utilizing enzymes play key roles in hair bundles, the mechanically sensitive organelles of sensory hair cells in the inner ear. We used a fluorescent ATP analog, EDA-ATP-Cy3 (Cy3-ATP), to label ATP-binding proteins in two different preparations of unfixed hair-cell stereocilia of the mouse. In the first preparation, we lightly permeabilized dissected cochleas, then labeled them with Cy3-ATP. Hair cells and their stereocilia remained intact, and stereocilia tips in rows 1 and 2 were labeled particularly strongly with Cy3-ATP. In many cases, vanadate (V

Indexed as

ActinsAdenosine TriphosphateAnimalsBridged Bicyclo Compounds, HeterocyclicCells, CulturedCytochalasin DHair Cells, AuditoryIndolesMiceMice, Inbred C57BLMyosinsStereociliaThiazolidinesVanadates3'(2')-O-(N-(2-((2-((1-ethyl-3,3-dimethyl-5-sulfoindol-2-yl)prop-2-enylidene)-3,3-dimethyl-5-sulfoindol-1-yl)amino)ethyl)carbamoyl)-adenosine triphosphateActinsAdenosine TriphosphateBridged Bicyclo Compounds, HeterocyclicCytochalasin DIndoleslatrunculin AMyosinsThiazolidinesVanadates

Identifiers

PMID34903829
PMCPMC8668996
OpenAlexW4200327696

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.