ArticleScientific reports2021
Oligonucleotide conjugated antibody strategies for cyclic immunostaining.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Automated cGMP optical labeling of FDA-approved antibodies for human use.Nature protocols · 2026Review
- Cyclic immunofluorescence platform using photocleavable linkers for direct antibody labeling enables cancer phenotyping.bioRxiv : the preprint server for biology · 2026Article
- Spatial proteomics in precision medicine: technologies, bioinformatics, and translational applications.Precision clinical medicine · 2026Review
- Comparative Genomic Hybridization (CGH) in Genotoxicology: From the Basics to Modern Approaches.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Antibody-oligonucleotide conjugates for spatial proteomics: principles, applications, and challenges.Acta biochimica et biophysica Sinica · 2025Article
- Article
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- Click Chemistry-Based Bioconjugation of Iron Oxide Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Selection of antibody-binding covalent aptamers.Communications chemistry · 2024Article
- Detection of neoplastic-immune hybrid cells with metastatic properties in uveal melanoma.Biomarker research · 2024Article
- A novel dual-release scaffold for fluorescent labels improves cyclic immunofluorescence.RSC chemical biology · 2024Article
- TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections.Scientific reports · 2024Article
- Fluorophore multimerization on a PEG backbone as a concept for signal amplification and lifetime modulation.Scientific reports · 2024Article
- Ultra high content analyses of circulating and tumor associated hybrid cells reveal phenotypic heterogeneity.Scientific reports · 2024Article
- Article
- Detection of neoplastic-immune hybrid cells with metastatic properties in uveal melanoma.Research square · 2023Article
- MicroMagnify: A Multiplexed Expansion Microscopy Method for Pathogens and Infected Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- High-plex immunofluorescence imaging and traditional histology of the same tissue section for discovering image-based biomarkers.Nature cancer · 2023Article
- Precancerous Lesions of the Head and Neck Region and Their Stromal Aberrations: Piecemeal Data.Cancers · 2023Review
- MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues.Research square · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
A number of highly multiplexed immunostaining and imaging methods have advanced spatial proteomics of cancer for improved treatment strategies. While a variety of methods have been developed, the most widely used methods are limited by harmful signal removal techniques, difficulties with reagent production and antigen sensitivity. Multiplexed immunostaining employing oligonucleotide (oligos)-barcoded antibodies is an alternative approach that is growing in popularity. However, challenges remain in consistent conjugation of oligos to antibodies with maintained antigenicity as well as non-destructive, robust and cost-effective signal removal methods. Herein, a variety of oligo conjugation and signal removal methods were evaluated in the development of a robust oligo conjugated antibody cyclic immunofluorescence (Ab-oligo cyCIF) methodology. Both non- and site-specific conjugation strategies were assessed to label antibodies, where site-specific conjugation resulted in higher retained binding affinity and antigen-specific staining. A variety of fluorescence signal removal methods were also evaluated, where incorporation of a photocleavable link (PCL) resulted in full fluorescence signal removal with minimal tissue disruption. In summary, this work resulted in an optimized Ab-oligo cyCIF platform capable of generating high dimensional images to characterize the spatial proteomics of the hallmarks of cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.