Evidence map›Paper›PMID 34902458›Full record

ArticleJournal of virological methods2022

Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic.

Gabriel Martín, Susana Rojo-Alba, Cristian Castelló-Abietar, Fátima Abreu-Salinas, Isabel Costales, Jose Antonio Boga, Santiago Melón, Marta Elena Álvarez-Argüelles

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Gabriel MartínServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain. Electronic address: gabrielmartinrguez1994@gmail.com.
Susana Rojo-AlbaServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Cristian Castelló-AbietarServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Fátima Abreu-SalinasServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Isabel CostalesServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Jose Antonio BogaServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Santiago MelónServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Marta Elena Álvarez-ArgüellesServicio de Microbiología, Hospital Universitario Central de Asturias (HUCA) and Grupo de Investigación Microbiología Traslacional, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Instituto de Investigación Sanitaria del Principado de Asturias · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Among the methods used to diagnose COVID-19, those based on genomic detection by q(RT)-PCR are the most sensitive. To perform these assays, a previous genome extraction of the sample is required. The dramatic increase in the number of SARS-CoV-2 detection assays has increased the demand for extraction reagents hindering the supply of commercial reagents. Homemade reagents and procedures could be an alternative. Nasopharyngeal samples were extracted by seven different methods as well as the automatic method MagNaPure96, to detect SARS-CoV-2. All protocols show sensitivity higher than 87 %, in comparison with reference method, for detecting SARS-CoV-2 as well as human β- globin. Our results support that these procedures, using common and cheap reagents, are effective to extract RNA (from SARS-CoV-2) or DNA (from human β-globin) genome from nasopharyngeal swabs. Furthermore, these procedures could be easily adopted by routine diagnostic laboratories to implement detection methods to help to fight against COVID-19 pandemic.

Indexed as

COVID-19HumansPandemicsRNA, ViralSARS-CoV-2Sensitivity and SpecificityRNA, ViralCOVID-19Genome extractionHeat extractionIn-house protocolSARS-CoV-2

Identifiers

PMID34902458
PMCPMC8662953
OpenAlexW4226108479

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.