Evidence map›Paper›PMID 34901889›Full record

ArticleSTAR protocols2021

Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue.

Tarlan Vatan, Jacqueline A Minehart, Chenghang Zhang, Vatsal Agarwal, Jerry Yang, Colenso M Speer

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.7field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Tarlan VatanDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Jacqueline A MinehartDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Chenghang ZhangDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Vatsal AgarwalDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Jerry YangDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
Colenso M SpeerDepartment of Biology, University of Maryland, College Park, MD 20742, USA.
University of Maryland, College Park · US

Funding

Synaptic Transmission in The Rod Pathway of the Mammalian RetinaR01EY017836 · NEI · UNIV OF MARYLAND, COLLEGE PARK · PI Jonathan B Demb, Anastas Popratiloff · 2007 to 2026
$7.2M
Neural Circuits and Synapses for Early Visual ProcessingR01EY014454 · NEI · YALE UNIVERSITY · PI DEMB, JONATHAN B · 2005 to 2022
$6.7M
A molecular connectomics platform for multi-scale analysis of activity-dependent synapse development and plasticityDP2MH125812 · NIMH · UNIV OF MARYLAND, COLLEGE PARK · PI SPEER, COLENSO MCNAUGHTON · 2020 to 2020
$2.2M
Functional Circuitry of Long-Range Connections in the RetinaR01EY029323 · NEI · YALE UNIVERSITY · PI DEMB, JONATHAN B · 2018 to 2021
$2.0M
NEI NIH HHS R01 EY014454NEI NIH HHS R01 EY017836NEI NIH HHS R01 EY029323NIMH NIH HHS DP2 MH125812
6 · The paper itself

Abstract

Here, we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models. For complete details on the use and execution of this protocol, please refer to Sigal et al. (2015).

Indexed as

RetinaAnimalsFemaleImaging, Three-DimensionalImmunohistochemistryMaleMiceSingle Molecule ImagingSynapsesCell BiologyMicroscopyNeuroscience

Identifiers

PMID34901889
PMCPMC8637648
OpenAlexW4200257700

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.