Production of PEGylated GCSF from Non-classical Inclusion Bodies Expressed in

Nguyen Thi My Trinh, Tran Linh Thuoc, Dang Thi Phuong Thao

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Article in Avicenna journal of medical biotechnology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

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3 authors at 2 institutions in 1 country.

Nguyen Thi My TrinhDepartment of Molecular and Environmental Biotechnology, University of Science, Ho Chi Minh City, Vietnam.

Tran Linh ThuocDepartment of Molecular and Environmental Biotechnology, University of Science, Ho Chi Minh City, Vietnam.

Dang Thi Phuong ThaoDepartment of Molecular and Environmental Biotechnology, University of Science, Ho Chi Minh City, Vietnam.

Vietnam National University Ho Chi Minh City · VNHo Chi Minh City University of Science · VN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe recombinant human granulocyte colony stimulating factor conjugated with polyethylene glycol (PEGylated GCSF) has currently been used as an efficient drug for the treatment of neutropenia caused by chemotherapy due to its long circulating half-life. Previous studies showed that Granulocyte Colony Stimulating Factor (GCSF) could be expressed as non-classical Inclusion Bodies (ncIBs), which contained likely correctly folded GCSF inside at low temperature. Therefore, in this study, a simple process was developed to produce PEGylated GCSF from ncIBs.

methodsBL21 (DE3)/pET-GCSF cells were cultured in the LiFlus GX 1.5

resultsPEGylated GCSF was obtained with high purity (∼97%) and was finally demonstrated as a form containing one GCSF molecule and one 5

conclusionThese results clearly indicate that the process developed in this study might be a potential and practical approach to produce PEGylated GCSF from ncIBs expressed in

Indexed as

Granulocyte colony stimulating factor (G-CSF)Inclusion bodiesPolyethylene glycol

Identifiers

PMID34900145

PMCPMC8606113

OpenAlexW4225564681

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LicenceCC BY-NC

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