ReviewFrontiers in plant science2021
New Technologies and Strategies for Grapevine Breeding Through Genetic Transformation.
Review in Frontiers in plant science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- Dissecting gene regulatory networks driving grape berry ripening using comparative systems biology across nonclimacteric fleshy fruits: the NAC-centered hierarchy revisited.Horticulture research · 2026Review
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
- High-efficiency callus induction from intact Camellia sinensis leaves via controlled incisions and its regulatory mechanisms.BMC plant biology · 2026Article
- Grapevine phyllosphere pan-metagenomics reveals pan-microbiome structure, diversity, and functional roles in downy mildew resistance.Microbiome · 2025Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- Genome Editing by Grafting.International journal of molecular sciences · 2025Review
- Inhibitory Effects of Garlic Extract on Hop Stunt Viroid in Micropropagated Grapevine Plantlets.The plant pathology journal · 2025Article
- Development and Applications of Somatic Embryogenesis in Grapevine (Plants (Basel, Switzerland) · 2024Review
- Article
- A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries.Protoplasma · 2024Article
- From buds to shoots: insights into grapevine development from the Witch's Broom bud sport.BMC plant biology · 2024Article
- Studies on Improving the Efficiency of Somatic Embryogenesis in Grapevine (Plants (Basel, Switzerland) · 2023Article
- CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding.International journal of molecular sciences · 2023Review
- Non-climacteric fruit development and ripening regulation: 'the phytohormones show'.Journal of experimental botany · 2023Review
- A simple and efficient protocol for transient transformation of sliced grape berries.Protoplasma · 2023Article
- Induction, Flavonoids Contents, and Bioactivities Analysis of Hairy Roots and True Roots ofMolecules (Basel, Switzerland) · 2023Article
- CRISPR-based resistance to grapevine virus A.Frontiers in plant science · 2023Article
- Boosting grapevine breeding for climate-smart viticulture: from genetic resources to predictive genomics.Frontiers in plant science · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Grapevine, as other woody perennials, has been considered a recalcitrant crop to produce transgenic plants. Since the production of transgenic and/or edited plants requires the ability to regenerate plants from transformed tissues, this step is often the biggest bottleneck in the process. The objective of this work is to review the state of the art technologies and strategies for the improvement of grapevine transformation and regeneration, focusing on three aspects: (i) problems associated with grapevine transformation; (ii) genes that promote grapevine regeneration; and (iii) vehicles for gene delivery. Concerning the first aspect, it is well documented that one of the main factors explaining the low success rate in obtaining transgenic plants is the regeneration process. After transgenic integration into receptor cells, tissue culture is required to regenerate transgenic seedlings from transformed cells. This process is time consuming and often requires the addition of environmentally damaging reagents (antibiotics and herbicides) to the culture medium to select transgenic plants. On the other hand, the expression of genes such as the so-called developmental regulators (DR), which induce specific development programs, can be used to avoid traditional tissue culture methods. The ectopic expression of specific combinations of DR in somatic cells has the potential to induce
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.