ReviewFrontiers in synaptic neuroscience2021
Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.
Review in Frontiers in synaptic neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- Increased Telomere Mobility in Progeria is Restored by Isoprenylcysteine Carboxyl Methyltransferase Inhibition.bioRxiv : the preprint server for biology · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- Optical Approaches to Dissect the Structure and Dynamics of the Synapse at Nanoscale Resolution.Advances in neurobiology · 2026Review
- Lysine demethylase 4A is a centrosome-associated protein required for centrosome integrity and genomic stability.The FEBS journal · 2026Article
- From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025Review
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.The journal of physical chemistry. B · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- Long axial-range double-helix point spread functions for 3D volumetric super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.Molecular psychiatry · 2024Review
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.bioRxiv : the preprint server for biology · 2024Article
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging.Neurophotonics · 2024Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The function of the neuronal synapse depends on the dynamics and interactions of individual molecules at the nanoscale. With the development of single-molecule super-resolution microscopy over the last decades, researchers now have a powerful and versatile imaging tool for mapping the molecular mechanisms behind the biological function. However, imaging of thicker samples, such as mammalian cells and tissue, in all three dimensions is still challenging due to increased fluorescence background and imaging volumes. The combination of single-molecule imaging with light sheet illumination is an emerging approach that allows for imaging of biological samples with reduced fluorescence background, photobleaching, and photodamage. In this review, we first present a brief overview of light sheet illumination and previous super-resolution techniques used for imaging of neurons and synapses. We then provide an in-depth technical review of the fundamental concepts and the current state of the art in the fields of three-dimensional single-molecule tracking and super-resolution imaging with light sheet illumination. We review how light sheet illumination can improve single-molecule tracking and super-resolution imaging in individual neurons and synapses, and we discuss emerging perspectives and new innovations that have the potential to enable and improve single-molecule imaging in brain tissue.
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