ArticleEvolutionary ecology research2019
Efficient CRISPR-Cas9 editing of major evolutionary loci in sticklebacks.
Article in Evolutionary ecology research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Testing vivo-morpholino mediated gene knockdown in threespine stickleback.bioRxiv : the preprint server for biology · 2026Article
- CRISPR screening reveals genetic regulators associated with the evolution of eye degeneration.bioRxiv : the preprint server for biology · 2025Article
- Ectodysplasin overexpression reveals spatiotemporally dynamic tooth formation competency in stickleback and zebrafish.Development (Cambridge, England) · 2025Article
- Molecular mechanisms of Eda-mediated adaptation to freshwater in threespine stickleback.Molecular ecology · 2025Article
- Ancient developmental genes underlie evolutionary novelties in walking fish.Current biology : CB · 2024Article
- The genetic basis of novel trait gain in walking fish.bioRxiv : the preprint server for biology · 2023Article
- Whole-genome Comparisons Identify Repeated Regulatory Changes Underlying Convergent Appendage Evolution in Diverse Fish Lineages.Molecular biology and evolution · 2023Article
- Ten years of marine evolutionary biology-Challenges and achievements of a multidisciplinary research initiative.Evolutionary applications · 2023Article
- Whole-genome comparisons identify repeated regulatory changes underlying convergent appendage evolution in diverse fish lineages.bioRxiv : the preprint server for biology · 2023Article
- Evolution of stickleback spines through independent cis-regulatory changes at HOXDB.Nature ecology & evolution · 2022Article
- Speciation and adaptation research meets genome editing.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- The ectodysplasin-A receptor is a candidate gene for lateral plate number variation in stickleback fish.G3 (Bethesda, Md.) · 2022Article
- Extreme intraspecific divergence in mitochondrial haplotypes makes the threespine stickleback fish an emerging evolutionary mutant model for mito-nuclear interactions.Frontiers in genetics · 2022Article
- Threespine Stickleback: A Model System For Evolutionary Genomics.Annual review of genomics and human genetics · 2021Review
- Longer or shorter spines: Reciprocal trait evolution in stickleback via triallelic regulatory changes inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Fitness maps to a large-effect locus in introduced stickleback populations.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Phosphorus limitation does not drive loss of bony lateral plates in freshwater stickleback (Gasterosteus aculeatus).Evolution; international journal of organic evolution · 2020Article
- Adaptation via pleiotropy and linkage: Association mapping reveals a complex genetic architecture within the sticklebackEvolution letters · 2020Article
- The power and limitations of gene expression pathway analyses toward predicting population response to environmental stressors.Evolutionary applications · 2020Article
- Out with the old, in with the new: Meiotic driving of sex chromosome evolution.Seminars in cell & developmental biologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundStickleback fish are widely used to study the genetic and ecological basis of phenotypic evolution. Although several major loci have now been identified that contribute to evolutionary differences between wild populations, further study of the phenotypes associated with particular genes and mutations has been limited by the difficulty of generating targeted mutations at precise locations in the stickleback genome. APPROACH AND
aimsWe compared different methods of expressing single-guide RNAs (sgRNAs) and Cas9 activity in fertilized stickleback eggs. We used an easily scored pigmentation gene (
resultsTargeted deletions were successfully induced at each target locus by co-injecting one-cell stage stickleback embryos with either
conclusionCRISPR-Cas9 approaches can be used to induce germline mutations in key evolutionary loci in sticklebacks. Targeted coding region mutations confirm an important role for
Indexed as
Identifiers
34899072PMC8664273W4206866113What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.