Evidence map›Paper›PMID 34897286›Full record

ArticleInternational journal of obesity (2005)2022

Pannexin 3 deletion reduces fat accumulation and inflammation in a sex-specific manner.

C Brent Wakefield, Vanessa R Lee, Danielle Johnston, Parastoo Boroumand, Nicolas J Pillon, Samar Sayedyahossein, Brooke L O'Donnell, Justin Tang, Rafael E Sanchez-Pupo, Kevin J Barr and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in International journal of obesity (2005), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Pannexins in the vasculature.American journal of physiology. Heart and circulatory physiology · 2025
    Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

C Brent WakefieldDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
Vanessa R LeeDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
Danielle JohnstonDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
Parastoo BoroumandCell Biology Program, The Hospital for Sick Children, Toronto, ON, M5G 0A4, Canada.
Nicolas J PillonCell Biology Program, The Hospital for Sick Children, Toronto, ON, M5G 0A4, Canada.ORCID http://orcid.org/0000-0003-1107-9490
Samar SayedyahosseinDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
Brooke L O'DonnellDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.ORCID http://orcid.org/0000-0001-6025-8300
Justin TangDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.ORCID http://orcid.org/0000-0002-9942-6655
Rafael E Sanchez-PupoDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.ORCID http://orcid.org/0000-0002-6970-2137
Kevin J BarrDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.
Robert GrosDepartment of Physiology and Pharmacology, University of Western Ontario, London, ON, N6A 5C1, Canada.
Lauren FlynnDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada.ORCID http://orcid.org/0000-0001-6227-0928
Nica M BorradaileDepartment of Physiology and Pharmacology, University of Western Ontario, London, ON, N6A 5C1, Canada.
Amira KlipCell Biology Program, The Hospital for Sick Children, Toronto, ON, M5G 0A4, Canada.
Frank BeierWestern's Bone and Joint Institute, The Dr. Sandy Kirkley Centre for Musculoskeletal Research, University Hospital, London, ON, N6G 2V4, Canada.
Silvia PenuelaDepartment of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, N6A 5C1, Canada. spenuela@uwo.ca.ORCID http://orcid.org/0000-0003-4829-5517
Western University · CAUniversity of Toronto · CA

Funding

CIHR 332438
6 · The paper itself

Abstract

backgroundPannexin 3 (PANX3) is a channel-forming glycoprotein that enables nutrient-induced inflammation in vitro, and genetic linkage data suggest that it regulates body mass index. Here, we characterized inflammatory and metabolic parameters in global Panx3 knockout (KO) mice in the context of forced treadmill running (FEX) and high-fat diet (HFD).

methodsC57BL/6N (WT) and KO mice were randomized to either a FEX running protocol or no running (SED) from 24 until 30 weeks of age. Body weight was measured biweekly, and body composition was measured at 24 and 30 weeks of age. Male WT and KO mice were fed a HFD from 12 to 28 weeks of age. Metabolic organs were analyzed for a panel of inflammatory markers and PANX3 expression.

resultsIn females there were no significant differences in body composition between genotypes, which could be due to the lack of PANX3 expression in female white adipose tissue, while male KOs fed a chow diet had lower body weight and lower fat mass at 24 and 30 weeks of age, which was reduced to the same extent as 6 weeks of FEX in WT mice. In addition, male KO mice exhibited significantly lower expression of multiple pro-inflammatory genes in white adipose tissue compared to WT mice. While on a HFD body weight differences were insignificant, multiple inflammatory genes were significantly different in quadriceps muscle and white adipose tissue resulting in a more anti-inflammatory phenotype in KO mice compared to WT. The lower fat mass in male KO mice may be due to significantly fewer adipocytes in their subcutaneous fat compared to WT mice. Mechanistically, adipose stromal cells (ASCs) cultured from KO mice grow significantly slower than WT ASCs.

conclusionPANX3 is expressed in male adult mouse adipose tissue and may regulate adipocyte numbers, influencing fat accumulation and inflammation.

Indexed as

Adipose TissueObesityAnimalsBody WeightDiet, High-FatFemaleInflammationMaleMiceMice, Inbred C57BLMice, Knockout

Identifiers

PMID34897286
OpenAlexW4226155448

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.