Evidence map›Paper›PMID 34894625›Full record

ArticleBiosensors & bioelectronics2022

Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform.

Changsheng He, Cailing Lin, Guosheng Mo, Binbin Xi, An An Li, Dongchao Huang, Yanbin Wan, Feng Chen, Yufeng Liang, Qingxia Zuo and 7 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 61 citations in OpenAlex.

  1. Article
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  6. Expanding the CRISPR/Cas toolkit: applications in proteomics and theranostics.Frontiers in bioengineering and biotechnology · 2025
    Review
  7. A Critical Review of the CRISPR-Cas Technology in the Detection of SARS-CoV-2 Variants.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 1 country.

Changsheng HeSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Cailing LinSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Guosheng MoSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Binbin XiSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
An An LiSchool of Public Health, Southern Medical University, Guangzhou, 510515, China.
Dongchao HuangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Yanbin WanSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Feng ChenSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Yufeng LiangSchool of Public Health, Southern Medical University, Guangzhou, 510515, China.
Qingxia ZuoSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Wanqing XuSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Dongyan FengSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Guanting ZhangSchool of Public Health, Sun Yat-sen University, Guangzhou, 510080, China.
Liya HanSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Changwen KeGuangdong Provincial Center for Disease Control and Prevention, Guangzhou, 511430, China.
Hongli DuSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.
Lizhen HuangSchool of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China. Electronic address: huanglzh@scut.edu.cn.
South China University of Technology · CNSouthern Medical University · CNGuangdong Provincial Center for Disease Control and Prevention · CNSun Yat-sen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns owing to higher transmission rates, disease severity, and escape from neutralizing antibodies. Rapid and accurate detection of SARS-CoV-2 variants provides crucial information concerning the outbreaks of SARS-CoV-2 variants and possible lines of transmission. This information is vital for infection prevention and control. We used a Cas12a-based RT-PCR combined with CRISPR on-site rapid detection system (RT-CORDS) platform to detect the key mutations in SARS-CoV-2 variants, such as 69/70 deletion, N501Y, and D614G. We used type-specific CRISPR RNAs (crRNAs) to identify wild-type (crRNA-W) and mutant (crRNA-M) sequences of SARS-CoV-2. We successfully differentiated mutant variants from wild-type SARS-CoV-2 with a sensitivity of 10

Indexed as

Biosensing TechniquesCOVID-19CRISPR-Cas SystemsHumansMutationSARS-CoV-269/70 deletionCRISPR/Cas12D614GN501YRT-CORDSSARS-CoV-2 variants

Identifiers

PMID34894625
PMCPMC8635686
OpenAlexW3216794941

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.