ArticlePlant physiology2022
Type 2C protein phosphatase clade D family members dephosphorylate guard cell plasma membrane H+-ATPase.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- LRS1 phosphorylated in response to blue light regulates stomatal opening.Journal of plant research · 2026Article
- Molecular Mechanisms and Experimental Strategies for Understanding Plant Drought Response.Plants (Basel, Switzerland) · 2026Review
- The multifaceted roles of PP2C phosphatases in plant growth, signaling, and responses to abiotic and biotic stresses.Plant communications · 2025Review
- Unravelling the dynamics of seed-stored mRNAs during seed priming.The New phytologist · 2025Article
- The protein kinase inhibitor, tyrphostin AG126, and its derivatives suppress phosphorylation of plasma membrane H+-ATPase and light-induced stomatal opening.Plant & cell physiology · 2025Article
- Protein Phosphatases MoPtc5, MoPtc1, and MoPtc2 Contribute to the Vegetative Growth, Stress Adaptation, and Virulence ofJournal of fungi (Basel, Switzerland) · 2025Article
- Origin and evolution of auxin-mediated acid growth.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Structural and functional characterization of genes PYL-PP2C-SnRK2s in the ABA signalling pathway of Cucurbita pepo.BMC genomics · 2024Article
- Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane HNature communications · 2024Article
- Phosphorylation of plasma membrane HNature communications · 2024Article
- A novel semi-dominant mutation inFrontiers in plant science · 2024Article
- PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice.Plant physiology · 2023Article
- Stomatal properties of Arabidopsis cauline and rice flag leaves and their contributions to seed production and grain yield.Journal of experimental botany · 2023Article
- Photosynthetic-Product-Dependent Activation of Plasma Membrane H+-ATPase and Nitrate Uptake in Arabidopsis Leaves.Plant & cell physiology · 2023Article
- SALT OVERLY SENSITIVE 1 is inhibited by clade D Protein phosphatase 2C D6 and D7 in Arabidopsis thaliana.The Plant cell · 2023Article
- Review
- Elevated COThe New phytologist · 2022Article
- Dephosphorylate to close: type 2C protein phosphatases regulate stomatal aperture.Plant physiology · 2022Article
- Height to first pod: A review of genetic and breeding approaches to improve combine harvesting in legume crops.Frontiers in plant science · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
Abstract
Plasma membrane (PM) H+-ATPase in guard cells is activated by phosphorylation of the penultimate residue, threonine (Thr), in response to blue and red light, promoting stomatal opening. Previous in vitro biochemical investigation suggested that Mg2+- and Mn2+-dependent membrane-localized type 2C protein phosphatase (PP2C)-like activity mediates the dephosphorylation of PM H+-ATPase in guard cells. PP2C clade D (PP2C.D) was later demonstrated to be involved in PM H+-ATPase dephosphorylation during auxin-induced cell expansion in Arabidopsis (Arabidopsis thaliana). However, it is unclear whether PP2C.D phosphatases are involved in PM H+-ATPase dephosphorylation in guard cells. Transient expression experiments using Arabidopsis mesophyll cell protoplasts revealed that all PP2C.D isoforms dephosphorylate the endogenous PM H+-ATPase. We further analyzed PP2C.D6/8/9, which display higher expression levels than other isoforms in guard cells, observing that pp2c.d6, pp2c.d8, and pp2c.d9 single mutants showed similar light-induced stomatal opening and phosphorylation status of PM H+-ATPase in guard cells as Col-0. In contrast, the pp2c.d6/9 double mutant displayed wider stomatal apertures and greater PM H+-ATPase phosphorylation in response to blue light, but delayed dephosphorylation of PM H+-ATPase in guard cells; the pp2c.d6/8/9 triple mutant showed similar phenotypes to those of the pp2c.d6/9 double mutant. Taken together, these results indicate that PP2C.D6 and PP2C.D9 redundantly mediate PM H+-ATPase dephosphorylation in guard cells. Curiously, unlike auxin-induced cell expansion in seedlings, auxin had no effect on the phosphorylation status of PM H+-ATPase in guard cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.