Evidence map›Paper›PMID 34894176›Full record

ArticleCytometry. Part B, Clinical cytometry2023

Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet.

Vincent H J van der Velden, Frank Preijers, Ulrika Johansson, Theresia M Westers, Alan Dunlop, Anna Porwit, Marie C Béné, Peter Valent, Jeroen Te Marvelde, Orianne Wagner-Ballon and 26 more

Abstract read
In one paragraph

Article in Cytometry. Part B, Clinical cytometry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

36 authors.

Vincent H J van der VeldenLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.ORCID 0000-0001-9457-3763
Frank PreijersDepartment of Laboratory Medicine - Laboratory for Hematology, Radboudumc, Nijmegen, The Netherlands.
Ulrika JohanssonLaboratory Medicine, SI-HMDS, University Hospitals Bristol and Weston NHS Foundation Trust, Bristol, UK.ORCID 0000-0003-0743-7400
Theresia M WestersDepartment of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.
Alan DunlopDepartment of Haemato-Oncology, Royal Marsden Hospital, Sutton, Surrey, UK.
Anna PorwitDepartment of Clinical Sciences, Division of Oncology And Pathology, Faculty of Medicine, Lund University, Lund, Sweden.ORCID 0000-0003-0709-2713
Marie C BénéHematology Biology, Nantes University Hospital and CRCINA, Nantes, France.ORCID 0000-0002-6569-7414
Peter ValentDepartment of Internal Medicine I, Division of Hematology & Hemostaseology, Medical University of Vienna, Vienna, Austria.
Jeroen Te MarveldeLaboratory Medical Immunology, Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Orianne Wagner-BallonDepartment of Hematology and Immunology; and Université Paris-Est Créteil, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Inserm U955, Créteil, France.ORCID 0000-0001-9071-6683
Uta OelschlaegelDepartment of Internal Medicine, University Hospital Carl-Gustav-Carus, Dresden, TU, Germany.
Leonie SaftDepartment of Clinical Pathology and Oncology, Karolinska University Hospital and Institute, Solna, Stockholm, Sweden.
Sharham KordastiComprehensive Cancer Centre, King's College London and Hematology Department, Guy's Hospital, London, UK.
Robin IrelandComprehensive Cancer Centre, King's College London and Hematology Department, Guy's Hospital, London, UK.
Eline CremersDepartment of Internal Medicine, Division of Hematology, Maastricht University Medical Center, AZ, Maastricht, The Netherlands.
Canan AlhanDepartment of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.
Carolien DuetzDepartment of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.
Willemijn HoboDepartment of Laboratory Medicine - Laboratory for Hematology, Radboudumc, Nijmegen, The Netherlands.
Nicolas ChapuisAssistance Publique-Hôpitaux de Paris. Centre-Université de Paris, Cochin Hospital, Laboratory of Hematology and Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France.ORCID 0000-0003-0601-3948
Michaela FontenayAssistance Publique-Hôpitaux de Paris. Centre-Université de Paris, Cochin Hospital, Laboratory of Hematology and Université de Paris, Institut Cochin, INSERM U1016, CNRS UMR8104, Paris, France.
Peter BettelheimDepartment of Internal Medicine, Ordensklinikum Linz Barmherzige Schwestern - Elisabethinen, Linz, Austria.
Lisa Eidenshink-BrodersenHematologics Inc, Seattle, WA, USA.
Patricia FontDepartment of Hematology, Hospital General Universitario Gregorio Marañon-IiSGM, Madrid, Spain.
Michael R LokenHematologics Inc, Seattle, WA, USA.
Sergio MatarrazCancer Research Center (IBMCC, USAL-CSIC), Department of Medicine and Cytometry Service, University of Salamanca, Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
Kiyoyuki OgataMetropolitan Research and Treatment Centre for Blood Disorders (MRTC Japan), Tokyo, Japan.
Alberto OrfaoCancer Research Center (IBMCC, USAL-CSIC), Department of Medicine and Cytometry Service, University of Salamanca, Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
Katherina PsarraImmunology Histocompatibility Department, Evangelismos Hospital, Athens, Greece.
Dolores SubiráFlow Cytometry Unit. Department of Hematology, Hospital Universitario de Guadalajara, Guadalajara, Spain.ORCID 0000-0002-9485-0529
Denise A WellsHematologics Inc, Seattle, WA, USA.
Matteo G Della PortaIRCCS Humanitas Research Hospital, Rozzano, Milan, Italy & Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy.
Kate BurburyDepartment of Haematology, Peter MacCallum Cancer Centre, University of Melbourne, Melbourne, Australia.
Frauke BellosMLL Munich Leukemia Laboratory, Munich, Germany.
Elisabeth WeißMLL Munich Leukemia Laboratory, Munich, Germany.
Wolfgang KernMLL Munich Leukemia Laboratory, Munich, Germany.
Arjan van de LoosdrechtDepartment of Hematology, Amsterdam UMC, location VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFlow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures.

methodsRecommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group.

resultsWe report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies.

conclusionsThese recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.

Indexed as

Myelodysplastic SyndromesBiological AssayFlow CytometryFluorescent DyesHumansReference StandardsFluorescent DyesELNflow cytometryMDSpre-analytic issues

Identifiers

PMID34894176
PMCPMC10078694

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.