ArticlePlant physiology2022
A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 49 citations in OpenAlex.
- Cross-stage single-cell and spatial metabolome analyses reveal periderm specialization and tanshinone biosynthesis in Salvia miltiorrhiza roots.The New phytologist · 2026Article
- bHLH130 mediates gibberellin signaling to regulate tanshinone biosynthesis inActa pharmaceutica Sinica. B · 2026Article
- The transcriptional regulatory network involved in periderm-specific tanshinones biosynthesis inActa pharmaceutica Sinica. B · 2026Article
- Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis.Plant molecular biology · 2026Article
- The Jasmonate-Responsive SmMPK3-SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2026Article
- Structure-activity relationships and molecular mechanisms of natural products in sepsis-associated organ dysfunction: a narrative review.Frontiers in pharmacology · 2026Review
- Diterpenoids in medicinal plants: structure, distribution, biological activities, biosynthesis, and bioengineering prospects.Frontiers in plant science · 2026Review
- Non-targeted metabolomics reveals tissue-specific metabolic profiling and antioxidant - related markers inFrontiers in plant science · 2026Article
- SmbZIP5 Integrates Abscisic and Jasmonic Acid Signalling to Positively Regulate Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2025Article
- Genome-wide identification of the SmPHR gene family in Salvia miltiorrhiza and SmPHR7-mediated response to phosphate starvation in Arabidopsis thaliana.Plant cell reports · 2025Article
- Biosynthesis and signal transduction of plant growth regulators and their effects on bioactive compound production in Salvia miltiorrhiza (Danshen).Chinese medicine · 2024Review
- The Smi-miR858a-Horticulture research · 2024Article
- Comparative transcriptomics of two Salvia subg. Perovskia species contribute towards molecular background of abietane-type diterpenoid biosynthesis.Scientific reports · 2024Article
- Transcriptome Analysis ofPlants (Basel, Switzerland) · 2024Article
- 2-oxoglutarate-dependent dioxygenases and BAHD acyltransferases drive the structural diversification of orobanchol in Fabaceae plants.Frontiers in plant science · 2024Article
- LcSAO1, an Unconventional DOXB Clade 2OGD Enzyme fromInternational journal of molecular sciences · 2023Article
- Identification of novel flavin-dependent monooxygenase from Strobilanthes Cusia reveals molecular basis of indoles' biosynthetic logic.BMC plant biology · 2023Article
- Chemical Constituents ofMetabolites · 2023Article
- Unveiling the spatial distribution and molecular mechanisms of terpenoid biosynthesis inHorticulture research · 2023Article
- Characteristics ofHorticulture research · 2023Article
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Authors and funding
17 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tanshinone ⅡA (TⅡA), a diterpene quinone with a furan ring, is a bioactive compound found in the medicinal herb redroot sage (Salvia miltiorrhiza Bunge), in which both furan and dihydrofuran analogs are present in abundance. Progress has been made recently in elucidating the tanshinone biosynthetic pathway, including heterocyclization of the dihydrofuran D-ring by cytochrome P450s; however, dehydrogenation of dihydrofuran to furan, a key step of furan ring formation, remains uncharacterized. Here, by differential transcriptome mining, we identified six 2-oxoglutarate-dependent dioxygenase (2-ODD) genes whose expressions corresponded to tanshinone biosynthesis. We showed that Sm2-ODD14 acts as a dehydrogenase catalyzing the furan ring aromatization. In vitro Sm2-ODD14 converted cryptotanshinone to TⅡA and thus was designated TⅡA synthase (SmTⅡAS). Furthermore, SmTⅡAS showed a strict substrate specificity, and repression of SmTⅡAS expression in hairy root by RNAi led to increased accumulation of total dihydrofuran-tanshinones and decreased production of furan-tanshinones. We conclude that SmTⅡAS controls the metabolite flux from dihydrofuran- to furan-tanshinones, which influences medicinal properties of S. miltiorrhiza.
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