ArticleMolecular biology and evolution2022
Loss of Timeless Underlies an Evolutionary Transition within the Circadian Clock.
Article in Molecular biology and evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 2 syntheses or guidelines pooled it, 57 citations in OpenAlex.
- Pooled it
- Genetic analysis of cryptochrome in insect magnetosensitivity.Frontiers in physiology · 2022Pooled it
- Knockout analysis of period and timeless and EGFP-based visualization of per-expressing clock cells in the cricket circadian clock.Zoological letters · 2026Article
- Revitalizing the "biology" in "chronobiology".Npj biological timing and sleep · 2026Review
- Review
- Circadian Control of Lipid Metabolism.Advances in experimental medicine and biology · 2026Review
- Phase response curve and RNA-sequencing demonstrate spiders' sensitivity to light and pinpoint candidate light-responsive genes.PloS one · 2026Article
- Circadian clock genes: Their influence on liver metabolism, disease development and treatment (Review).Molecular medicine reports · 2026Review
- Coevolution ofiScience · 2025Article
- A Head-Specific Transcriptomic Study Reveals Key Regulatory Pathways for Winter Diapause in the Mosquito Culex pipiens.Archives of insect biochemistry and physiology · 2025Article
- Biological rhythms: Living your life, one half-day at a time.Npj biological timing and sleep · 2025Review
- Deciphering a Beetle Clock: Individual and Sex-Dependent Variation in Daily Activity Patterns.Journal of biological rhythms · 2024Article
- The Free-Running Circasemilunar Period Is Determined by Counting Circadian Clock Cycles in the Marine MidgeJournal of biological rhythms · 2024Article
- Drosophila ezoana uses morning and evening oscillators to adjust its rhythmic activity to different daylengths but only the morning oscillator to measure night length for photoperiodic responses.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Article
- The circadian and photoperiodic clock of the pea aphid.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Light pollution disrupts circadian clock gene expression in two mosquito vectors during their overwintering dormancy.Scientific reports · 2024Article
- SP3-induced Timeless transcription contributes to cell growth of lung adenocarcinoma cells.PloS one · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- c-Jun N-terminal kinase signaling in aging.Frontiers in aging neuroscience · 2024Review
- Circadian Rhythms of Locomotor Activity Mediated byInsects · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most organisms possess time-keeping devices called circadian clocks. At the molecular level, circadian clocks consist of transcription-translation feedback loops (TTFLs). Although some components of the negative TTFL are conserved across the animals, important differences exist between typical models, such as mouse and the fruit fly. In Drosophila, the key components are PERIOD (PER) and TIMELESS (TIM-d) proteins, whereas the mammalian clock relies on PER and CRYPTOCHROME (CRY-m). Importantly, how the clock has maintained functionality during evolutionary transitions between different states remains elusive. Therefore, we systematically described the circadian clock gene setup in major bilaterian lineages and identified marked lineage-specific differences in their clock constitution. Then we performed a thorough functional analysis of the linden bug Pyrrhocoris apterus, an insect species comprising features characteristic of both the Drosophila and the mammalian clocks. Unexpectedly, the knockout of timeless-d, a gene essential for the clock ticking in Drosophila, did not compromise rhythmicity in P. apterus, it only accelerated its pace. Furthermore, silencing timeless-m, the ancestral timeless type ubiquitously present across animals, resulted in a mild gradual loss of rhythmicity, supporting its possible participation in the linden bug clock, which is consistent with timeless-m role suggested by research on mammalian models. The dispensability of timeless-d in P. apterus allows drawing a scenario in which the clock has remained functional at each step of transition from an ancestral state to the TIM-d-independent PER + CRY-m system operating in extant vertebrates, including humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.