ArticleMolecular biology and evolution2022
Selection for Cell Yield Does Not Reduce Overflow Metabolism in Escherichia coli.
Article in Molecular biology and evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Biocalorimetry for the biotechnological use of natural and synthetic macromolecules.Applied microbiology and biotechnology · 2026Review
- Rapid growth phenotype of carbapenem-resistant Enterobacter cloacae: growth fitness, stability, and mechanistic insights.BMC genomics · 2026Article
- The effect of substrate and product transfer between polydisperse microdroplets on biological high-throughput screens.Scientific reports · 2026Article
- Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli.Metabolic engineering · 2026Article
- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- Review
- Translating microbial kinetics into quantitative responses and testable hypotheses using Kinbiont.Nature communications · 2025Article
- Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria.Journal of bacteriology · 2025Review
- Minimizing acetate formation from overflow metabolism inFrontiers in bioengineering and biotechnology · 2024Article
- Prevalent emergence of reciprocity among cross-feeding bacteria.ISME communications · 2022Article
- Heme A Synthase Deficiency Affects the Ability ofInternational journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Overflow metabolism is ubiquitous in nature, and it is often considered inefficient because it leads to a relatively low biomass yield per consumed carbon. This metabolic strategy has been described as advantageous because it supports high growth rates during nutrient competition. Here, we experimentally evolved bacteria without nutrient competition by repeatedly growing and mixing millions of parallel batch cultures of Escherichia coli. Each culture originated from a water-in-oil emulsion droplet seeded with a single cell. Unexpectedly we found that overflow metabolism (acetate production) did not change. Instead, the numerical cell yield during the consumption of the accumulated acetate increased as a consequence of a reduction in cell size. Our experiments and a mathematical model show that fast growth and overflow metabolism, followed by the consumption of the overflow metabolite, can lead to a higher numerical cell yield and therefore a higher fitness compared with full respiration of the substrate. This provides an evolutionary scenario where overflow metabolism can be favorable even in the absence of nutrient competition.
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