ArticleScience advances2021
Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Robust thermometry-imaging down to sub-micrometer or millisecond-resolution by fluorescence lifetime microscopy allows for additional acquisition of multiple imaging channels.Scientific reports · 2026Article
- Phase-targeting rapid cryofixation of the beating heart and histological analysis unveil contractile state-dependent sarcomere dynamics.Scientific reports · 2026Article
- Determination of factors that allow cryogenic nanoscopy with high power illumination without devitrification.PloS one · 2026Article
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Article
- Cryogenic single-molecule fluorescence imaging.BMB reports · 2025Review
- Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging.Science advances · 2024Article
- Model-Based Optimization of Solid-Supported Micro-Hotplates for Microfluidic Cryofixation.Micromachines · 2024Article
- The EGFR phosphatase RPTPγ is a redox-regulated suppressor of promigratory signaling.The EMBO journal · 2023Article
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Authors and funding
6 authors.
Funding
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Abstract
Imaging molecular patterns in cells by fluorescence micro- or nanoscopy has the potential to relate collective molecular behavior to cellular function. However, spatial and spectroscopic resolution is fundamentally limited by motional blur caused by finite photon fluxes and photobleaching. At physiological temperatures, photochemical reactivity does not only limit imaging at multiple scales but is also toxic to biochemical reactions that maintain cellular organization. Here, we present cryoprotectant-free ultrarapid cryo-arrest directly on a multimodal fluorescence microscope that preserves the out-of-equilibrium molecular organization of living cells. This allows the imaging of dynamic processes before cryo-arrest in combination with precise molecular pattern determination at multiple scales within the same cells under cryo-arrest. We both experimentally and theoretically show that ultrarapid cryo-arrest overcomes the fundamental resolution barrier imposed by motional blur and photochemical reactivity, enabling observation of native molecular distributions and reaction patterns that are not resolvable at physiological temperatures.
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Registered trials
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