ArticleScientific reports2021
Alpha-diversity and microbial community structure of the male urinary microbiota depend on urine sampling method.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Alterations in urinary microbiota composition and function in men with Asthenozoospermia: a case-control study.Basic and clinical andrology · 2026Article
- Microbial community structure and source contribution to the early neovaginal microbiota following penile inversion vaginoplasty.Frontiers in cellular and infection microbiology · 2026Article
- Methodological bias shapes the interpretation of the urinary microbiome in low-biomass systems.Frontiers in microbiology · 2026Review
- Impact of the urinary microbiome on urinary tract infection progression.Central European journal of urology · 2026Review
- Urinary microbiota in bladder cancer: insights into pathogenesis, diagnosis, and therapeutic potential.Frontiers in cellular and infection microbiology · 2026Review
- The urinary microbiome: the next frontier of bacterial ecology.Journal of bacteriology · 2025Review
- Body mass index and benign prostatic hyperplasia correlate with urinary microbiome diversity and lower urinary tract symptoms in men.Communications medicine · 2025Article
- Short- and mid-term temporal variability of the human urinary microbiota: a prospective observational cohort study.BMC microbiology · 2025Observational
- The role of urinary microbiota in primary and recurrent bladder cancer: insights from a propensity score matching study.BMC cancer · 2025Article
- Urinary tract infections: pathogenesis, host susceptibility and emerging therapeutics.Nature reviews. Microbiology · 2025Review
- Exploring urinary microbiome: insights into neurogenic bladder and improving management of urinary tract infections.Frontiers in cellular and infection microbiology · 2025Review
- Comparison of the urinary microbiome in men who have sex with men with and without Chlamydia trachomatis infection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Changes in the Urinary Microbiome After Transurethral Resection of Non-muscle-Invasive Bladder Cancer: Insights from a Prospective Observational Study.Annals of surgical oncology · 2024Observational
- Survey of the infant male urobiome and genomic analysis of Actinotignum spp.NPJ biofilms and microbiomes · 2023Article
- The impact of urine collection method on canine urinary microbiota detection: a cross-sectional study.BMC microbiology · 2023Article
- Human Male Genital Tract Microbiota.International journal of molecular sciences · 2023Review
- Clinical relevance of expanded quantitative urine culture in health and disease.Frontiers in cellular and infection microbiology · 2023Review
- Determination of the Optimal Bacterial DNA Extraction Method to Explore the Urinary Microbiota.International journal of molecular sciences · 2022Article
- Localization and potential role of prostate microbiota.Frontiers in cellular and infection microbiology · 2022Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Considerable variation exists in the methodology of urinary microbiota studies published so far including the cornerstone of any biomedical analysis: sample collection. The aim of this study was to compare the urinary microbiota of first-catch voided urine (FCU), mid-stream voided urine (MSU) and aseptically catheterised urine in men and define the most suitable urine sampling method. Forty-nine men (mean age 71.3 years) undergoing endoscopic urological procedures were enrolled in the study. Each of them contributed three samples: first-catch urine (FCU), mid-stream urine (MSU) and a catheterised urine sample. The samples were subjected to next-generation sequencing (NGS, n = 35) and expanded quantitative urine culture (EQUC, n = 31). Using NGS, Bacteroidetes, Firmicutes, and Proteobacteria were the most abundant phyla in our population. The most abundant genera (in order of relative abundance) included: Prevotella, Veillonella, Streptococcus, Porphyromonas, Campylobacter, Pseudomonas, Staphylococcus, Ezakiella, Escherichia and Dialister. Eighty-two of 105 samples were dominated by a single genus. FCU, MSU and catheterised urine samples differed significantly in three of five alpha-diversity measures (ANOVA, p < 0.05): estimated number of operational taxonomic units, Chao1 and abundance-based coverage estimators. Beta-diversity comparisons using the PIME method (Prevalence Interval for Microbiome Evaluation) resulted in clustering of urine samples according to the mode of sampling. EQUC detected cultivable bacteria in 30/31 (97%) FCU and 27/31 (87%) MSU samples. Only 4/31 (13%) of catheterised urine samples showed bacterial growth. Urine samples obtained by transurethral catheterisation under aseptic conditions seem to differ from spontaneously voided urine samples. Whether the added value of a more exact reflection of the bladder microbiota free from urethral contamination outweighs the invasiveness of urethral catheterisation remains to be determined.
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