Evidence map›Paper›PMID 34887464›Full record

ArticleScientific reports2021

Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay.

Jae Jin Lee, Sung Hyun Kim, Keon Ah Lee, Kimleng Chuon, Kwang-Hwan Jung, Doseok Kim

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Jae Jin LeeDepartment of Physics, Sogang University, Seoul, Korea.
Sung Hyun KimDepartment of BioNanoScience Kavli Institute of Nanoscience of Delft University of Technology, Delft, The Netherlands.
Keon Ah LeeDepartment of Life Science, Sogang University, Seoul, Korea.
Kimleng ChuonDepartment of Life Science, Sogang University, Seoul, Korea.
Kwang-Hwan JungDepartment of Life Science, Sogang University, Seoul, Korea.
Doseok KimDepartment of Physics, Sogang University, Seoul, Korea. doseok@sogang.ac.kr.
Sogang University · KRDelft University of Technology · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na

Indexed as

Nucleic Acid ConformationAnabaenaCyclizationDNADNA-Binding ProteinsGene Expression Regulation, BacterialModels, BiologicalSensory RhodopsinsSodium ChlorideSpectrum AnalysisDNADNA-Binding ProteinsSensory RhodopsinsSodium Chloride

Identifiers

PMID34887464
PMCPMC8660804
OpenAlexW4200549699

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.