Evidence map›Paper›PMID 34885732›Full record

ArticleMolecules (Basel, Switzerland)2021

Expression, Purification and Characterization of a Novel Hybrid Peptide CLP with Excellent Antibacterial Activity.

Junhao Cheng, Marhaba Ahmat, Henan Guo, Xubiao Wei, Lulu Zhang, Qiang Cheng, Jing Zhang, Junyong Wang, Dayong Si, Yueping Zhang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. The future of recombinant host defense peptides.Microbial cell factories · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Junhao ChengLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.ORCID 0000-0002-1260-1802
Marhaba AhmatLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.ORCID 0000-0001-6635-9313
Henan GuoLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Xubiao WeiLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Lulu ZhangLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Qiang ChengLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Jing ZhangLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Junyong WangLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Dayong SiLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Yueping ZhangLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Rijun ZhangLaboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Animal Nutrition

Funding

National Natural Science Foundation of China 31572442National Natural Science Foundation of China 32100156the National Key Research and Development Program of China 2018YFD0500600
6 · The paper itself

Abstract

CLP is a novel hybrid peptide derived from CM4, LL37 and TP5, with significantly reduced hemolytic activity and increased antibacterial activity than parental antimicrobial peptides. To avoid host toxicity and obtain high-level bio-production of CLP, we established a His-tagged SUMO fusion expression system in

Indexed as

Anti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesBacteriaCathelicidinsEscherichia coliHemolysisHumansPeptidesRecombinant Fusion ProteinsAnti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesCAMP protein, humanCathelicidinsCM4 peptide, Bombyx moriPeptidesRecombinant Fusion Proteinsantibacterial activityantimicrobial peptideEscherichia colifusion expressionhybrid peptide

Identifiers

PMID34885732
PMCPMC8659006
OpenAlexW4200086955

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.