Evidence map›Paper›PMID 34884636›Full record

ReviewInternational journal of molecular sciences2021

Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination.

Loredana-Mirela Lupu, Pascal Wiegand, Daria Holdschick, Delia Mihoc, Stefan Maeser, Stephan Rawer, Friedemann Völklein, Ebrahim Malek, Frederik Barka, Sascha Knauer and 6 more

Open access · goldAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Loredana-Mirela LupuCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Pascal WiegandCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Daria HoldschickCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Delia MihocCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Stefan MaeserCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Stephan RawerCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Friedemann VölkleinDepartment of Engineering & Institute for Microtechnologies (IMTECH), RheinMain University, 65428 Rüsselsheim am Main, Germany.
Ebrahim MalekCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Frederik BarkaSunchrom GmbH, Industriestr. 18, 61381 Friedrichsdorf, Germany.
Sascha KnauerSulfotools GmbH, Bahnhofsplatz 1, 65428 Rüsselsheim am Main, Germany.ORCID 0000-0002-4282-3983
Christina UthSulfotools GmbH, Bahnhofsplatz 1, 65428 Rüsselsheim am Main, Germany.
Julia HennermannDepartment of Pediatrics, Universitätsmedizin Mainz, 55130 Mainz, Germany.
Wolfgang KleinekofortCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Andreas HahnDepartment of Child Neurology, Justus-Liebig-University Giessen, Feulgenstraße 10-12, 35389 Giessen, Germany.
Günes BarkaSunchrom GmbH, Industriestr. 18, 61381 Friedrichsdorf, Germany.
Michael PrzybylskiCentre for Analytical Biochemistry and Biomedical Mass Spectrometry (AffyMSLifeChem), and Steinbeis Transfer Centre for Biopolymer Analysis and Biomedical Mass Spectrometry, Marktstrasse 29, 65428 Rüsselsheim am Main, Germany.
Steinbeis Foundation · DEJohannes Gutenberg University Mainz · DEJustus-Liebig-Universität Gießen · DERheinMain University of Applied Sciences · DE

Funding

European Union IRSES Project No. 269256Research Ninistery State Hessen LOEWE Programme HA-696/19-16
6 · The paper itself

Abstract

Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (K

Indexed as

AnimalsAntibodiesAntibody AffinityAptamers, NucleotideBiosensing TechniquesEpitopesHumansSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSurface Plasmon ResonanceAntibodiesAptamers, NucleotideEpitopesaffinity determinationchip-MALDI-mass spectrometrycross-immunoreactivityDNA aptamersepitope structure determinationinterleukin-8monoclonalmyoglobinpolyclonal protein antibodiesproteolytic epitope extractionSPR

Identifiers

PMID34884636
PMCPMC8657952
OpenAlexW3215487399

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.