Evidence map›Paper›PMID 34884574›Full record

ArticleInternational journal of molecular sciences2021

Stability of Erythrocyte-Derived Nanovesicles Assessed by Light Scattering and Electron Microscopy.

Darja Božič, Matej Hočevar, Matic Kisovec, Manca Pajnič, Ljubiša Pađen, Marko Jeran, Apolonija Bedina Zavec, Marjetka Podobnik, Ksenija Kogej, Aleš Iglič and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
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  12. Interaction between MicroalgaeInternational journal of molecular sciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Darja BožičLaboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Matej HočevarDepartment of Physics and Chemistry of Materials, Institute of Metals and Technology, SI-1000 Ljubljana, Slovenia.
Matic KisovecDepartment of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia.ORCID 0000-0003-4131-2177
Manca PajničLaboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Ljubiša PađenLaboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Marko JeranLaboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.ORCID 0000-0001-8064-4970
Apolonija Bedina ZavecDepartment of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia.
Marjetka PodobnikDepartment of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia.ORCID 0000-0001-6786-9737
Ksenija KogejFaculty of Chemistry and Chemical Technology, University of Ljubljana, SI-1000 Ljubljana, Slovenia.ORCID 0000-0002-6291-1032
Aleš IgličLaboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, SI-1000 Ljubljana, Slovenia.ORCID 0000-0002-7895-343X
Veronika Kralj-IgličLaboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.ORCID 0000-0002-7496-2005
University of Ljubljana · SINational Institute of Chemistry · SIInstitute of Metals and Technology · SI

Funding

EU Commission Ves4us, 801338Slovenian Research Agency P2-0232, P3-0388, P1-0201, P1-0391, P2-0123, L3-2621
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are gaining increasing amounts of attention due to their potential use in diagnostics and therapy, but the poor reproducibility of the studies that have been conducted on these structures hinders their breakthrough into routine practice. We believe that a better understanding of EVs stability and methods to control their integrity are the key to resolving this issue. In this work, erythrocyte EVs (hbEVs) were isolated by centrifugation from suspensions of human erythrocytes that had been aged in vitro. The isolate was characterised by scanning (SEM) and cryo-transmission electron microscopy (cryo-TEM), flow cytometry (FCM), dynamic/static light scattering (LS), protein electrophoresis, and UV-V spectrometry. The hbEVs were exposed to various conditions (pH (4-10), osmolarity (50-1000 mOsm/L), temperature (15-60 °C), and surfactant Triton X-100 (10-500 μM)). Their stability was evaluated by LS by considering the hydrodynamic radius (

Indexed as

Dynamic Light ScatteringErythrocytesExtracellular VesiclesHumansMicroscopy, Electroncellular vesiclescryo-electron microscopy of extracellular vesiclesextracellular vesicle reference materiallight scatteringnanovesiclesscanning electron microscopy of extracellular vesiclesvesicle characterizationvesicle shapevesicle stability

Identifiers

PMID34884574
PMCPMC8657685
OpenAlexW3215325225

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.