Splice-Variant Knock-Out of TGFβ Receptors Perturbates the Proteome of Ovarian Carcinoma Cells.

Liora Jacobs Catane, Ofra Moshel, Yoav Smith, Ben Davidson, Reuven Reich

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 50% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

The Role of EMT-Related lncRNAs in Ovarian Cancer.International journal of molecular sciences · 2023
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Liora Jacobs CataneInstitute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.

Ofra MoshelCore Research Facility, Institute for Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.

Yoav SmithGenomic Data Analysis Unit, The Hebrew University of Jerusalem-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.

Ben DavidsonDepartment of Pathology, Oslo University Hospital, Norwegian Radium Hospital, N-0310 Oslo, Norway.

Reuven ReichInstitute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.ORCID 0000-0003-1684-5967

Hebrew University of Jerusalem · ILOslo University Hospital · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to analyze the biological role of different transforming growth factor-β (TGFβ) receptor splice variants in ovarian carcinoma (OC). Specific receptor variant knockouts (KO) were prepared using the CRISPR/Cas9 genome editing system in two OC cell lines, TβRI variant 1 (TβRIv1) KO in ES-2 cells and TβRII variant 1 (TβRIIv1) KO in OVCAR-8 cells. Control and KO cells were compared by proteomic analysis, functional tests, analysis of epithelial-mesenchymal transition (EMT) drivers, and Western blot of signaling proteins. Proteomic analysis revealed significant changes in protein pathways in the KO cells. TβRIv1 KO resulted in a significant reduction in both cellular motility and invasion, while TβRIIv1 KO significantly reduced cellular motility and increased Reactive Oxygen Species (ROS) production. Both receptor variant KOs reduced MET protein levels. Of the EMT drivers, a significant decrease in TWIST protein expression, and increase in SNAIL protein and

Indexed as

Alternative SplicingCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticGene Knockout TechniquesHumansNuclear ProteinsOvarian NeoplasmsProtein Interaction MapsProteomicsProto-Oncogene Proteins c-metReactive Oxygen SpeciesReceptor, Transforming Growth Factor-beta Type IReceptor, Transforming Growth Factor-beta Type IIMALAT1 long non-coding RNA, humanMET protein, humanNuclear ProteinsProto-Oncogene Proteins c-metReactive Oxygen SpeciesReceptor, Transforming Growth Factor-beta Type IReceptor, Transforming Growth Factor-beta Type IIRNA, Long NoncodingSNAI1 protein, humanSnail Family Transcription FactorsTGFBR1 protein, humanTGFBR2 protein, humanTWIST1 protein, humanTwist-Related Protein 1CRISPR/Cas9 KO’sovarian carcinomaproteomicsreceptor splice variantsTGFβtransforming growth factor-β

Identifiers

PMID34884451

DOI10.3390/ijms222312647

PMCPMC8657817

OpenAlexW3216950345

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.