ArticleEvolution; international journal of organic evolution2022
Unraveling patterns of disrupted gene expression across a complex tissue.
Article in Evolution; international journal of organic evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Comparative single-nucleus transcriptomics reveals asymmetric evolution of the Drosophila male and female germlines.PLoS biology · 2026Article
- Evolution of Gene Expression Across Functional Regions of the Mouse Placenta.Genome biology and evolution · 2026Article
- Conserved Gene Expression Plasticity in Development Is More Pervasive Than Expression Divergence Between Species of Caenorhabditis Nematodes.Evolution & development · 2025Article
- Hybridization and gene expression: Beyond differentially expressed genes.Molecular ecology · 2025Review
- Dichotomous transcriptome divergence at the cell-type level between thebioRxiv : the preprint server for biology · 2025Article
- Intraspecific gene regulation in cis- and trans.Evolution; international journal of organic evolution · 2025Review
- Different complex regulatory phenotypes underlie hybrid male sterility in divergent rodent crosses.Genetics · 2025Article
- Different complex regulatory phenotypes underlie hybrid male sterility in divergent rodent crosses.bioRxiv : the preprint server for biology · 2024Article
- Do genetic loci that cause reproductive isolation in the lab inhibit gene flow in nature?Evolution; international journal of organic evolution · 2024Article
- Gene expression and alternative splicing contribute to adaptive divergence of ecotypes.Heredity · 2024Article
- Sex-biased gene expression at single-cell resolution: cause and consequence of sexual dimorphism.Evolution letters · 2023Article
- The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice.Genetics · 2022Article
- Extensive transgressive gene expression in testis but not ovary in the homoploid hybrid Italian sparrow.Molecular ecology · 2022Article
- Stage-specific disruption of X chromosome expression during spermatogenesis in sterile house mouse hybrids.G3 (Bethesda, Md.) · 2022Article
- Molecular Evolution across Mouse Spermatogenesis.Molecular biology and evolution · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Whole tissue RNASeq is the standard approach for studying gene expression divergence in evolutionary biology and provides a snapshot of the comprehensive transcriptome for a given tissue. However, whole tissues consist of diverse cell types differing in expression profiles, and the cellular composition of these tissues can evolve across species. Here, we investigate the effects of different cellular composition on whole tissue expression profiles. We compared gene expression from whole testes and enriched spermatogenesis populations in two species of house mice, Mus musculus musculus and M. m. domesticus, and their sterile and fertile F1 hybrids, which differ in both cellular composition and regulatory dynamics. We found that cellular composition differences skewed expression profiles and differential gene expression in whole testes samples. Importantly, both approaches were able to detect large-scale patterns such as disrupted X chromosome expression, although whole testes sampling resulted in decreased power to detect differentially expressed genes. We encourage researchers to account for histology in RNASeq and consider methods that reduce sample complexity whenever feasible. Ultimately, we show that differences in cellular composition between tissues can modify expression profiles, potentially altering inferred gene ontological processes, insights into gene network evolution, and processes governing gene expression evolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.