Evidence map›Paper›PMID 34879708›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2022

Single-Cell RNA Sequencing and Assay for Transposase-Accessible Chromatin Using Sequencing Reveals Cellular and Molecular Dynamics of Aortic Aging in Mice.

Wenhui Xie, Yilang Ke, Qinyi You, Jing Li, Lu Chen, Dang Li, Jun Fang, Xiaofeng Chen, Yuanyuan Zhou, Liangwan Chen and 1 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 39 citations in OpenAlex.

  1. Review
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  8. Decoding Vascular Senescence: Cellular Insights and Therapeutic Strategies.International journal of biological sciences · 2026
    Review
  9. Article
  10. Distinguishing subtypes of endothelial cells in the mouse aorta.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
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  17. Vascular smooth muscle cell-derived SOFrontiers in pharmacology · 2025
    Article
  18. Review
  19. Review
  20. Tissue-resident C1q + macrophages exert anti-aging potential through the Sirt1 pathway.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Wenhui Xie *Department of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Yilang Ke *Fujian Key Laboratory of Vascular Aging, Fujian Medical University, Fuzhou, China (W.X., Y.K., D.L., H.H.).
Qinyi You *Department of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Jing LiDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Lu ChenDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Dang LiFujian Key Laboratory of Vascular Aging, Fujian Medical University, Fuzhou, China (W.X., Y.K., D.L., H.H.).
Jun FangDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Xiaofeng ChenDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Yuanyuan ZhouDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Liangwan ChenDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).
Huashan HongDepartment of Geriatrics, Department of Cardiac Surgery, and Department of Cardiology, Fujian Heart Disease Center, Fujian Medical University Union Hospital, Fuzhou, China (W.X., Q.Y., J.L., L.C., J.F., X.C., Y.Z., L.C., H.H.).ORCID 0000-0001-8332-0066
Fujian Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe impact of vascular aging on cardiovascular diseases has been extensively studied; however, little is known regarding the cellular and molecular mechanisms underlying age-related vascular aging in aortic cellular subpopulations. Approach and Results: Transcriptomes and transposase-accessible chromatin profiles from the aortas of 4-, 26-, and 86-week-old C57/BL6J mice were analyzed using single-cell RNA sequencing and assay for transposase-accessible chromatin sequencing. By integrating the heterogeneous transcriptome and chromatin accessibility data, we identified cell-specific TF (transcription factor) regulatory networks and open chromatin states. We also determined that aortic aging affects cell interactions, inflammation, cell type composition, dysregulation of transcriptional control, and chromatin accessibility. Endothelial cells 1 have higher gene set activity related to cellular senescence and aging than do endothelial cells 2. Moreover, construction of senescence trajectories shows that endothelial cell 1 and fibroblast senescence is associated with distinct TF open chromatin states and an mRNA expression model.

conclusionsOur data provide a system-wide model for transcriptional and epigenetic regulation during aortic aging at single-cell resolution.

Indexed as

AgingTranscriptomeAnimalsAortaCardiovascular DiseasesChromatinChromatin Immunoprecipitation SequencingGene Regulatory NetworksMiceMice, Inbred C57BLMolecular Dynamics SimulationSequence Analysis, RNATranscription FactorsTransposasesChromatinTranscription FactorsTransposasesaortaendothelial cellsfibroblastsinflammationtranscriptome

Identifiers

PMID34879708
OpenAlexW4200296738

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.