ArticleDevelopment (Cambridge, England)2021
Visualizing polymeric components that define distinct root barriers across plant lineages.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Local adjustment of exodermis to nutrient deficiency in crop species.Frontiers in plant science · 2026Article
- Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy.Scientific reports · 2025Article
- Mangrove-Derived Endophytic Bacteria Enhance Growth, Yield, and Stress Resilience in Rice.International journal of molecular sciences · 2025Article
- A rapid staining method for the detection of suberin lamellae in the root endodermis and exodermis.Plant biotechnology (Tokyo, Japan) · 2025Article
- Article
- Microscopy and spatial-metabolomics identify tissue-specific metabolic pathways uncovering salinity and drought tolerance mechanisms in Avicennia marina and Phoenix dactylifera roots.Scientific reports · 2025Article
- Regulation and function of a polarly localized lignin barrier in the exodermis.Nature plants · 2025Article
- Integrated metabolomic and transcriptomic analysis reveals the role of root phenylpropanoid biosynthesis pathway in the salt tolerance of perennial ryegrass.BMC plant biology · 2024Article
- An atlas of Brachypodium distachyon lateral root development.Biology open · 2024Article
- The Arabidopsis SGN3/GSO1 receptor kinase integrates soil nitrogen status into shoot development.The EMBO journal · 2024Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A micro RNA mediates shoot control of root branching.Nature communications · 2023Article
- Article
- Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum.Plant physiology · 2022Article
- Tissue-specific regulation of lipid polyester synthesis genes controlling oxygen permeation intoProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The making of suberin.The New phytologist · 2022Review
- Multi-Omics Analyses on Maya-Land Tomatoes Shed Light on Plant Strategies to Thrive in High Temperatures.Physiologia plantarumArticle
- Sclerified Cork Outperforms the Exodermis: Root Water Permeability Decreases in the Soil-To-Canopy Transition of the Aroid Vine Epipremnum aureum.Physiologia plantarumArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrophobic cell wall depositions in roots play a key role in plant development and interaction with the soil environment, as they generate barriers that regulate bidirectional nutrient flux. Techniques to label the respective polymers are emerging, but are efficient only in thin roots or sections. Moreover, simultaneous imaging of the barrier constituents lignin and suberin remains problematic owing to their similar chemical compositions. Here, we describe a staining method compatible with single- and multiphoton confocal microscopy that allows for concurrent visualization of primary cell walls and distinct secondary depositions in one workflow. This protocol permits efficient separation of suberin- and lignin-specific signals with high resolution, enabling precise dissection of barrier constituents. Our approach is compatible with imaging of fluorescent proteins, and can thus complement genetic markers or aid the dissection of barriers in biotic root interactions. We further demonstrate applicability in deep root tissues of plant models and crops across phylogenetic lineages. Our optimized toolset will significantly advance our understanding of root barrier dynamics and function, and of their role in plant interactions with the rhizospheric environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.